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A robust potency assay highlights significant donor variation of human mesenchymal stem/progenitor cell immune modulatory capacity and extended radio-resistance

Ketterl, Nina ; Brachtl, Gabriele ; Schuh, Cornelia ; Bieback, Karen ; Schallmoser, Katharina ; Reinisch, Andreas ; Strunk, Dirk

In: Stem Cell Research & Therapy, 6 (2015), Nr. 236. pp. 1-11. ISSN 1757-6512

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Download (3MB) | Lizenz: Creative Commons LizenzvertragA robust potency assay highlights significant donor variation of human mesenchymal stem/progenitor cell immune modulatory capacity and extended radio-resistance by Ketterl, Nina ; Brachtl, Gabriele ; Schuh, Cornelia ; Bieback, Karen ; Schallmoser, Katharina ; Reinisch, Andreas ; Strunk, Dirk underlies the terms of Creative Commons Attribution 3.0 Germany

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Abstract

The inherent immunomodulatory capacity of mesenchymal stem/progenitor cells (MSPCs) encouraged initiation of multiple clinical trials. Release criteria for therapeutic MSPCs cover identity, purity and safety but appropriate potency assessment is often missing. Reports on functional heterogeneity of MSPCs created additional uncertainty regarding donor and organ/source selection. We established a robust immunomodulation potency assay based on pooling responder leukocytes to minimize individual immune response variability. Comparing various MSPCs revealed significant potency inconsistency and generally diminished allo-immunosuppression compared to dose-dependent inhibition of mitogenesis. Gamma-irradiation to block unintended MSPC proliferation did not prohibit chondrogenesis and osteogenesis in vivo, indicating the need for alternative safety strategies.

Item Type: Article
Journal or Publication Title: Stem Cell Research & Therapy
Volume: 6
Number: 236
Publisher: BioMed Central
Place of Publication: London
Date Deposited: 08 Feb 2016 13:59
Date: 2015
ISSN: 1757-6512
Page Range: pp. 1-11
Faculties / Institutes: Medizinische Fakultät Mannheim > Institut für Transfusionsmedizin und Immunologie
Subjects: 570 Life sciences
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