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Osseointegrative effect of rhBMP-2 covalently bound on a titan-plasma-spray-surface after modification with chromosulfuric acid in a large animal bone gap-healing model with the Göttingen minipig

Lingner, Manuel ; Seidling, Roland ; Lehmann, Lars Johannes ; Mauermann, Eckhard ; Obertacke, Udo ; Schwarz, Markus Ludiwg Rupert

In: Journal of Orthopaedic Surgery and Research, 13 (2018), Nr. 219. pp. 1-13. ISSN 1749-799X

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Download (2MB) | Lizenz: Creative Commons LizenzvertragOsseointegrative effect of rhBMP-2 covalently bound on a titan-plasma-spray-surface after modification with chromosulfuric acid in a large animal bone gap-healing model with the Göttingen minipig by Lingner, Manuel ; Seidling, Roland ; Lehmann, Lars Johannes ; Mauermann, Eckhard ; Obertacke, Udo ; Schwarz, Markus Ludiwg Rupert underlies the terms of Creative Commons Attribution 3.0 Germany

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Abstract

Background: Bone morphogenetic proteins play an important role as osseointegrative factors. It is used widely in orthopedic research and surgery to enhance the osseointegrative potential of implants, e.g., in spinal fusion or alveolar socket augmentation. The aim of the present study was to investigate the benefit of rhBMP-2 on a titan plasma spray (TPS) layer after a special modification with chromosulfuric acid (CSA) at different postoperative times, regarding osseoconduction and osseoinduction.

Methods: We allocated 27 Göttinger minipigs into three groups consisting of nine animals each. They received four dumbbell-shaped implants in the metaphyseal parts of the femora. The implants had a TPS surface with (CSA group) and without a CSA treatment (TPS group). The former received an additional layer of BMP-2 (BMP-2 group). For the assessment of osseointegration after healing periods of 4, 8, and 12 weeks, histomorphometry was applied to undecalcified specimens after staining according to Masson-Goldner. An intravital labeling with different fluorochromes was used in the gap model. A multivariable analysis with repeated measurement design was performed for statistical evaluation.

Results: We observed several statistical differences in a three-way ANOVA. The comparison between the BMP-2 and the TPS group (two-way ANOVA) showed statistically significant differences in terms of the osseoinduction (osteoid volume), and pronounced for the osseoconduction (bone and osteoid ongrowth), in favor of the BMP-2 group. In the pairwise comparison between BMP-2 and CSA (two-way ANOVA), no statistical significance occurred. The intravital staining with tetracycline, calcein green, and xylenol orange revealed no considerable differences between the groups.

Conclusion: BMP-2, covalently bound on a CSA-treated TPS surface, has positive effects on the osseointegration in the large animal bone gap-healing model over the observation period of 12 weeks.

Item Type: Article
Journal or Publication Title: Journal of Orthopaedic Surgery and Research
Volume: 13
Number: 219
Publisher: Biomed Central
Place of Publication: London
Date Deposited: 25 Oct 2018 07:00
Date: 2018
ISSN: 1749-799X
Page Range: pp. 1-13
Faculties / Institutes: Medizinische Fakultät Mannheim > Orthopädische Klinik
Subjects: 610 Medical sciences Medicine
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