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The Role of Rab GTPases in the Endolysosomal System of S.cerevisiae

Markgraf, Daniel Frank

German Title: Die Rolle von RabGTPasen im Endolysosomalen System von S.cerevisiae

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Abstract

The evolution of the eucaryotic cell was accompanied by the development of an elaborate endomembrane system. Transport between intracellular compartments is mediated by vesicular carriers that constantly bud from donor membranes and fuse with target compartments. RabGTPases together with tethering factors mediate first specific contact between membranes destined fusion. This study focuses on the interplay between the endosomal RabGTPase Vps21 and the endosomal tethering complex, CORVET. The CORVET subunit Vps8 is the direct effector subunit and mediates binding to the GTP-form of Vps21, whereas the subunit Vps3 binds the GDP-form of Vps21. Vps8 and Vps21 functionally interact to mediate late endosomal tethering events. Furthermore, Vps8 and Vps21, together with the two other CORVET subunits, Vps3 and Vps16 were shown to be the minimal molecular requirement for late endosomal tethering.The results presented here indicate that the sequential recruitment of Vps8 to Vps21-positive late endosomes initiates tethering and leads to further assembly of the CORVET complex that dictates successive fusion events.

Translation of abstract (English)

The evolution of the eucaryotic cell was accompanied by the development of an elaborate endomembrane system. Transport between intracellular compartments is mediated by vesicular carriers that constantly bud from donor membranes and fuse with target compartments. RabGTPases together with tethering factors mediate first specific contact between membranes destined fusion. This study focuses on the interplay between the endosomal RabGTPase Vps21 and the endosomal tethering complex, CORVET. The CORVET subunit Vps8 is the direct effector subunit and mediates binding to the GTP-form of Vps21, whereas the subunit Vps3 binds the GDP-form of Vps21. Vps8 and Vps21 functionally interact to mediate late endosomal tethering events. Furthermore, Vps8 and Vps21, together with the two other CORVET subunits, Vps3 and Vps16 were shown to be the minimal molecular requirement for late endosomal tethering.The results presented here indicate that the sequential recruitment of Vps8 to Vps21-positive late endosomes initiates tethering and leads to further assembly of the CORVET complex that dictates successive fusion events.

Item Type: Dissertation
Supervisor: Brunner, Prof. Michael
Date of thesis defense: 8 December 2008
Date Deposited: 09 Dec 2008 14:49
Date: 2008
Faculties / Institutes: Service facilities > Centre for Organismal Studies Heidelberg (COS)
Subjects: 570 Life sciences
Controlled Keywords: Membrantransport, Endosom, Vakuole
Uncontrolled Keywords: RabGTPase , Tethering FaktorenVesicular transport , RabGTPase , Tethering factor
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