Directly to content
  1. Publishing |
  2. Search |
  3. Browse |
  4. Recent items rss |
  5. Open Access |
  6. Jur. Issues |
  7. DeutschClear Cookie - decide language by browser settings

Anti-infective and cytotoxic properties of Bupleurum marginatum

Ashour, Mohamed L ; Readi, Mahmoud Z ; Hamoud, Razan ; Eid, Safaa Y ; Ahmady, Sherweit H ; Nibret, Endalkachew ; Herrmann, Florian ; Youns, Mahmoud ; Tahrani, Ahmed ; Kaufmann, Dorothea ; Wink, Michael

In: Chinese Medicine, 9 (2014), Nr. 4. pp. 1-10. ISSN 1749-8546

[thumbnail of 13020_2013_Article_199.pdf]
Preview
PDF, English - main document
Download (547kB) | Lizenz: Creative Commons LizenzvertragAnti-infective and cytotoxic properties of Bupleurum marginatum by Ashour, Mohamed L ; Readi, Mahmoud Z ; Hamoud, Razan ; Eid, Safaa Y ; Ahmady, Sherweit H ; Nibret, Endalkachew ; Herrmann, Florian ; Youns, Mahmoud ; Tahrani, Ahmed ; Kaufmann, Dorothea ; Wink, Michael underlies the terms of Creative Commons Attribution 3.0 Germany

Citation of documents: Please do not cite the URL that is displayed in your browser location input, instead use the DOI, URN or the persistent URL below, as we can guarantee their long-time accessibility.

Abstract

Bupleurum marginatum Wall. ex DC (Apiaceae) is a perennial herb widely used in traditional Chinese and Kampo medicine for the treatment of various infectious diseases. The biological activities of B. marginatum have not been fully investigated. This study aims to investigate the antitrypanosomal, antimicrobial and antiviral activities of methanol (ME) and dichloromethane (DCM) extracts of B. marginatum aerial parts and the ability of both extracts to inhibit the growth of different cancer cell lines. Methods Phytochemical characterization of the extracts was performed by LC-MS profiling. The antitrypanosomal activity was evaluated using the resazurin method. The antimicrobial activity was assessed using agar diffusion and microdilution methods, and the minimum inhibitory concentration (MIC) values were determined. The antiviral activity was determined for 6.25, 12.5, and 50 μg/mL doses using a plaque reduction assay. Cytotoxicity was investigated in eight cancer cell lines (Caco-2, CCL-81, CCRF-CEM, COS-7, HL-60, MIA PaCa-2, MCF-7, and PANC-1) using the MTT assay and the caspase 3/7 activity was determined over the range of 62.5–1000 μg/mL. Results Phytochemical analyses resulted in the characterization of 15 components, mainly flavonoids and lignans. The DCM extract showed significant antitrypanosomal activity (IC50: 36.21 μg/mL) and moderate activity against Streptococcus pyogenes (MIC value: 0.25 mg/mL). At a dose of 12.5 μg/mL, the DCM extract inhibited 73.6% of the plaque production by hepatitis A virus. CCRF-CEM cells were the most sensitive to both extracts (IC50: 12.5–22.7 μg/mL). The cytotoxicity was mediated by induction of apoptosis (19-fold increase in the cellular caspase 3/7 level after treatment with the DCM extract at 1 mg/mL). Conclusions ME and DCM extract of B. marginatum showed anti-infective and antiproliferative effects.

Document type: Article
Journal or Publication Title: Chinese Medicine
Volume: 9
Number: 4
Publisher: BioMed Central
Place of Publication: London
Date Deposited: 13 May 2015 08:30
Date: 2014
ISSN: 1749-8546
Page Range: pp. 1-10
Faculties / Institutes: Fakultät für Ingenieurwissenschaften > Institute of Pharmacy and Molecular Biotechnology
DDC-classification: 570 Life sciences
About | FAQ | Contact | Imprint |
OA-LogoDINI certificate 2013Logo der Open-Archives-Initiative