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Abstract

Cholangiocellular carcinoma (CCC) is a rare tumor entity and the availability of systemic treatment is limited. Better knowledge of the tumor physiology might lead to an improved treatment. However, basic research is dependent on the availability of cell lines. As there are only few CCC cell lines disposable, we aimed to isolate new CCC cell lines from surgical samples. The cell line was established from a 59-year-old Caucasian male patient who suffered from malignant pleural effusion of a distal cholangiocellular carcinoma (CCC). The cell line was established by explant culture and further characterized with immunohistochemistry and Western blotting. We tested sensitivity towards commonly used chemotherapeutics. Immunohistochemistry and Western blotting showed expression of tumor markers typical for CCC cell lines. Analysis of sensitivity towards chemotherapeutics revealed that Irinotecan was the only agent with significant effect on cell proliferation. In conclusion the new cell line displays the typical characteristics of a CCC cell line. This new cell line might help to add new insights into this tumor entity and thereby help to find better treatment options for patients with CCC.