<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Mimicking the Cellular Environment: effects of elastic nanopatterned substrates on integrin-mediated cellular interactions"^^ . "In this work, the influence of environmental parameters on cellular behavior has been investigated. Therefore, an artifcial substrate system, according to the biophysical and biochemical properties of the extracellular matrix in connective tissues, has been developed. The Young’s moduli E of poly(ethylene glycol)-diacrylate (PEG-DA) substrates span more than four orders of magnitude (0.6 kPa < E < 6 MPa). Since PEG-DA substrates are protein repellent, they were decorated by quasi hexagonally ordered, extended gold nanoparticle arrays, manufactured by block copolymer micellar nanolithography (BCMN). To provide bioactivity in terms of cell adhesion c(RGDfK) peptide, which is specific for alphaV beta3 integrins, was immobilized on the nanoparticles. The interparticle spacing and, hence, spacing of integrin binding sites L could be precisely tuned, independently of the substrate rigidity, between 20 nm and 160 nm. We investigated the behavior of fibroblasts as a function of changes within this two-dimensional parameters space (L; E ). To this end, cell spreading area and cell-substrate interaction forces were determined by phase contrast microscopy and single cell force spectroscopy (SCFS), respectively. The experiments revealed two tactile set points, thresholds in cellular sensing behavior, at E = 8 kPa and L = 70 nm, after 6, 12, and 24 hours of adhesion, respectively. Moreover, according to the hierarchical phase model in cellular behavior, elasticity was identified to be the dominant parameter in cellular sensing processes. Thus, this work provides an important contribution to the understanding of cell-cell and cell-matrix adhesion. "^^ . "2009" . . . . . . . . "Ilia"^^ . "Louban"^^ . "Ilia Louban"^^ . . . . . . "Mimicking the Cellular Environment: effects of elastic nanopatterned substrates on integrin-mediated cellular interactions (PDF)"^^ . . . "Diss_Ilia_Louban_2009.pdf"^^ . . . "Mimicking the Cellular Environment: effects of elastic nanopatterned substrates on integrin-mediated cellular interactions (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Mimicking the Cellular Environment: effects of elastic nanopatterned substrates on integrin-mediated cellular interactions (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Mimicking the Cellular Environment: effects of elastic nanopatterned substrates on integrin-mediated cellular interactions (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Mimicking the Cellular Environment: effects of elastic nanopatterned substrates on integrin-mediated cellular interactions (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Mimicking the Cellular Environment: effects of elastic nanopatterned substrates on integrin-mediated cellular interactions (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #10342 \n\nMimicking the Cellular Environment: effects of elastic nanopatterned substrates on integrin-mediated cellular interactions\n\n" . "text/html" . . . "530 Physik"@de . "530 Physics"@en . .