<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Biochemical Characterization of the Drosophila Polycomb protein dSfmbt"^^ . "Polycomb group (PcG) proteins are transcriptional regulators that maintain the repression of a large set of developmental control genes. PcG proteins form distinct multiprotein complexes: PhoRC (Pho Repressive Complex), PRC1 (Polycomb Repressive Complex 1), PRC2 (Polycomb Repressive Complex 2) and its variant Pcl-PRC2. These complexes repress target genes by modifying their chromatin. PcG chromatin modifications are thought to provide a memory that permits the transcriptional OFF state to be maintained in a heritable manner. PcG protein complexes assemble at specific cis-regulatory sequences called Polycomb Response Elements (PREs). Although all PcG complexes are targeted to PREs, only the PhoRC subunit Pho has sequencespecific DNA binding activity. The mechanism by which PRC1 or PRC2 are targeted and tethered at PREs is only poorly understood. During my Ph. D. studies, I performed a biochemical characterization of dSfmbt, the protein that together with Pho forms PhoRC. Using a Tandem Affinity Purification strategy, I purified proteins associated with dSfmbt. These purified complexes contained not only dSfmbt and Pho but also the histone deacetylase Rpd3, the histone chaperone NAP1, the chromatin binding protein HP1b, and an uncharacterized protein, CG3363. This is further supported by the observation that in addition to the Pho and dSfmbt also Rpd3 is bound to PREs of PcG target genes. dSfmbt forms a stable complex with the PRC1 subunit Scm in vitro and these two proteins are bound at PREs of PcG target genes. Using genetic interaction assays, I found that Scm and dSfmbt act in a highly synergistic manner to repress PcG target genes in vivo during Drosophila development. Taken together, these studies thus suggest that the PhoRC complex comprises not only Pho and dSfmbt but also additional chromatinmodifying and chromatin-binding subunits. The molecular and functional interactions between dSfmbt and Scm underscore the central role of dSfmbt as a molecular adaptor between the DNA-binding Pho subunit and PRC1."^^ . "2009" . . . . . . . . "Raquel"^^ . "Vidal Matos"^^ . "Raquel Vidal Matos"^^ . . . . . . "Biochemical Characterization of the Drosophila Polycomb protein dSfmbt (PDF)"^^ . . . "Raquel_Vidal_Matos_Dissertation.pdf"^^ . . . "Biochemical Characterization of the Drosophila Polycomb protein dSfmbt (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Biochemical Characterization of the Drosophila Polycomb protein dSfmbt (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Biochemical Characterization of the Drosophila Polycomb protein dSfmbt (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Biochemical Characterization of the Drosophila Polycomb protein dSfmbt (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Biochemical Characterization of the Drosophila Polycomb protein dSfmbt (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #10684 \n\nBiochemical Characterization of the Drosophila Polycomb protein dSfmbt\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .