TY  - GEN
KW  - HV-CMOSHV-CMOS
AV  - public
A1  - König, Kai Lars
TI  - CMOS-Based Peptide Arrays
N2  - CMOS - Based Peptide Arrays Peptide arrays are an important tool in proteomics and peptidomics, allowing a large number of peptides to be synthesized on a common support and exposed to a solution of target molecules in parallel. In particle-based synthesis, the amino acids for in situ synthesis of peptides are transported to synthesis loci in solid particles and released upon melting, allowing an increase in density over liquid-based systems. This thesis focuses on the development of application-specific high voltage integrated circuits for electrostatic deposition of charged amino acid particles and their integration into a combinatorial peptide synthesis system.  Transfer of amino acid particles from the aerosol to synthesis loci on the chip surface was investigated for a pixel pitch between 45 µm and 100 µm, and compatibility between the chips, particle transfer and the poly(ethylene glycol)methacrylate - based surface modifi¬cations was established. The first combinatorial syntheses on CMOS chips were performed with over 16,000 distinct synthesis sites per chip, at a density of 10,000 spots per cm2, which is a 25-fold increase over the 400 spots per cm2 currently available on laser-printed glass slides. For FLAG and HA peptide epitopes, immonostaining showed regular spots of comparable signal intensity over the whole chip area. 
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/10911/
Y1  - 2010///
ID  - heidok10911
ER  -