TY  - GEN
A1  - Helwa, Reham
TI  - Functional studies on the galectin-4 promoter and its use for establishing a transcription factors array assay
N2  - In the present study, I profiled the expression pattern of different stages of six colorectal cancer and adenoma cell lines (SW1116, SW480, SW620, Co115, KM20L2, and LT97 in comparison to normal colon cell line CCD-18co). In our expression profiling data, we have found that galectin-4 was among the genes that are significantly upregulated in LT97 and KM20L2. We investigated galectin-4 upregulation as a signature of bad prognosis in colorectal cancer cell lines. In addition, we identified one possible mechanism of galectin-4 upregulation which is associated with a twin SNPs in the upstream sequence and the first intron. From the sequencing results of 115 patients, 26 out of the 115 (22.6%) were found to have the two SNPs together (ss217320370 and rs73933062), while the other patients did not carry any of these two SNPs. Therefore, we firstly showed that ss217320370 C>A at position 11572034 (a novel SNP was identified in the present study) and G>A at position 11571652 (rs73933062) are always together in the same individual (twin SNPs). Since the twin SNPs could potentially be associated with galectin-4 upregulation via deletion and insertion of new transcription factor binding sites, the twin SNPs may have medical impact in colorectal cancer patient. In parallel, another project was carried out to establish transcription factor protein. In conclusion, upregulation of galectin-4 was found to be associated with bad prognosis of colorectal cancer. In addition, two SNPs (ss217320370 and rs73933062) were found to be associated with galectin-4 upregulation, which might be referred to the changing in the binding sequence of the regulatory elements. Using galectin-4 promoter with the twin SNPs was useful in setting up TFs-array to detect DNA-protein interactions, since the microarray result was concordant with pull down-mass spectrometry analysis of galectin-4 promoter.
ID  - heidok11368
AV  - public
KW  - galectin-4 
KW  -  colorectal cancer 
KW  -  transcription factor-array 
KW  -  DNA-protein interaction
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/11368/
Y1  - 2010///
ER  -