%0 Generic
%A Wagner, Jennifer
%D 2011
%F heidok:12602
%K quality control , peptide synthesis , image processing , surface analysis
%R 10.11588/heidok.00012602
%T Quality control for peptide chip array production
%U https://archiv.ub.uni-heidelberg.de/volltextserver/12602/
%X This work establishes quality control for selective deposition of tribo-electrically charged micro-particles containing pre-activated amino acids onto a CMOS-chip in order to achieve high peptide densities in a modified Merrifield peptide synthesis. It encompasses the setup and implementation of an image processing supported auto- mated assessment of the particle depositions based on the analysis of the particles used, modelling the particle transfer out of an aerosol, deducing optimised param- eters and finding a surface analysis experiment that allows for semi-quantitative spatially resolved detection of amino acids coupled to the support in the microm- eter range. Furthermore, a flexible stand-alone interface to the control program of the automated system is implemented.