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primary topic: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes

On the spatial organization of cell organelles and diffusion of proteins in organelle membranes
type: Article
abstract: In the first part of this thesis, we study the diffusive motion of the (un)folded transmembrane protein VSVG in the endoplasmic reticulum (ER). Unfolded VSVG interacts with the ER’s quality control machinery. These interactions are probed by Fluorescence Correlation Spectroscopy in vivo. We find that both, folded and unfolded VSVG show anomalous diffusion, however, the unfolded protein has a significantly stronger anomaly. Our experimental data and accompanying simulations suggest that parts of the quality control oligomerize unfolded VSVG, leading to a more anomalous/obstructed diffusion. The difference in anomaly subsides when unfolded VSVG is in a complex with its chaperone calnexin. Hence, calnexin dissolves the oligomers and thus prevents ER poisoning. The second part of this work is dedicated to the influence of cell’s shape on its internal organization. We find that organelle positions show large variations in ensembles of equally shaped cells, hence challenging the idea of a ”standard cell”. However, depending on cell geometry and symmetry, the centrosome shows a preferential direction of displacement from the cell center. Simulations that include pushing forces of microtubules on the centrosome and the repulsion of the nucleus by the cell membrane explain this localization.
date: 2011
status: published
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (PDF)
has document: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (Other)
subject: 530 Physik

On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (PDF)
type: Document
has file: thesis.pdf
has part: thesis.pdf

Nina Isabelle Malchus
type: Person
given name: Nina Isabelle
family name: Malchus

On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (Other)
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is version of: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (PDF)
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On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (Other)
type: Document
is version of: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (PDF)
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On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (Other)
type: Document
is version of: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (PDF)
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On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (Other)
type: Document
is version of: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (PDF)
is volatile version of: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (PDF)
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On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (Other)
type: Document
is version of: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes (PDF)
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primary topic: On the spatial organization of cell organelles and diffusion of proteins in organelle membranes

530 Physik
type: Concept
preferred label: 530 Physics @en