eprintid: 12692
rev_number: 15
eprint_status: archive
userid: 1
dir: disk0/00/01/26/92
datestamp: 2011-11-21 12:04:44
lastmod: 2016-01-09 17:46:48
status_changed: 2012-08-15 09:02:42
type: preprint
metadata_visibility: show
creators_name: Friedmann, Elfriede
creators_name: Pfeifer, Andrea C.
creators_name: Neumann, Rebecca
creators_name: Klingmüller, Ursula
creators_name: Rannacher, Rolf
title: Interaction between Experiment, Modeling and Simulation of Spatial Aspects in the JAK2/STAT5 Signaling Pathway
ispublished: pub
subjects: ddc-510
divisions: i-110400
divisions: i-999999
keywords: JAK2/STAT5 Signalweg, Reaktions-Diffusionsgleichungen, ParameterschätzungJAK2/STAT5 Signaling Pathway, Reaction Diffusion Equations, Parameter Estimation
cterms_swd: Helmholtz Alliance on Systems Biology (SBCancer, Submodule V.7), FORSYS-ViroQuant (#0313923)
abstract: Fundamental progress in systems biology can only be achieved if experimentalists and theoreticians closely collaborate. Mathematical models cannot be formulated precisely without deep knowledge of the experiments while complex biological systems can often not be understood fully without mathematical interpretation of the dynamic processes involved. In this article, we describe how these two approaches can be combined to gain new insights on one of the most extensively studied signal transduction pathways, the Janus kinase (JAK)/ signal transducer and activator of transcription (STAT) pathway. We focus on the parameters of a model describing how STAT proteins are transported from the membrane to the nucleus where STATs regulate gene expression. We discuss which parameters can be measured experimentally in different cell types and how the unknown parameters are estimated, what the limits of these techniques and how accurate the determinations are.
abstract_translated_text: Fundamental progress in systems biology can only be achieved if experimentalists and theoreticians closely collaborate. Mathematical models cannot be formulated precisely without deep knowledge of the experiments while complex biological systems can often not be understood fully without mathematical interpretation of the dynamic processes involved. In this article, we describe how these two approaches can be combined to gain new insights on one of the most extensively studied signal transduction pathways, the Janus kinase (JAK)/ signal transducer and activator of transcription (STAT) pathway. We focus on the parameters of a model describing how STAT proteins are transported from the membrane to the nucleus where STATs regulate gene expression. We discuss which parameters can be measured experimentally in different cell types and how the unknown parameters are estimated, what the limits of these techniques and how accurate the determinations are.
abstract_translated_lang: eng
date: 2010
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00012692
ppn_swb: 1651063672
own_urn: urn:nbn:de:bsz:16-opus-126925
language: eng
bibsort: FRIEDMANNEINTERACTIO2010
full_text_status: public
citation:   Friedmann, Elfriede ; Pfeifer, Andrea C. ; Neumann, Rebecca ; Klingmüller, Ursula ; Rannacher, Rolf  (2010) Interaction between Experiment, Modeling and Simulation of Spatial Aspects in the JAK2/STAT5 Signaling Pathway.  [Preprint]     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/12692/1/FriedmannPfeifer10.pdf