<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Molecular and Cellular Analysis of the Nematostella vectensis Cnidom"^^ . "Cnidaria is the sister group of Bilateria and thus phylogenetically at the base of the Metazoa. The characteristic trait of the phylum is the presence of nematocysts or cnidae. These unique extrusive organelles are used for prey capture, feeding, defense and partly locomotion. Cnidae or nematocysts consist of a capsule wall, a tubule with or without spines, and an opercular structure. They are produced in specialized cells (nematocytes) as secretory products in a giant post-Golgi vesicle. These stinging organelles can resist extremely high pressure and their discharge is one of the fastest mechanisms in animal kingdom. In the last decade the sea anemone Nematostella vectensis (Anthozoa) became an emerging model organism alongside Hydra (Hydrozoa) for developmental and evolutionary biology. Nevertheless basic data on histology and the nematocyst repertoire (cnidom) of Nematostella vectensis are still missing. In my thesis, I characterized the Nematostella vectensis cnidom based on morphological classification. For that matter an isolation technique for intact capsules was established and the different nematocysts were analyzed using electron and light microscopy. The three capsule types (basitrichous haplonema, microbasic mastigophores and spirocysts) were quantitatively analyzed along the body axis and in the different developmental stages. Besides the morphological characterization, three molecular capsule components, minicollagen 1, 3 and 4 were isolated and in situ hybridization was performed to see their expression patterns. Antibody stainings revealed a partly abundant and partly nematocyst specific patterns of the minicollagens. The second part of the work was focused on the cellular identification of nematocysts in Nematostella vectensis. Thus, all cell types were characterized qualitatively and a cell cycle analysis was performed. Nematocytes were identified to be cuboidal epithelial cells using antibody stainings on macerates. Hence, the nematocysts in Nematostella vectensis have an epithelial origin and not as in Hydra a neuronal. "^^ . "2011" . . . . . . . . "Claudia"^^ . "Zenkert"^^ . "Claudia Zenkert"^^ . . . . . . "Molecular and Cellular Analysis of the Nematostella vectensis Cnidom (PDF)"^^ . . . "dissertation_claudia_zenkert.pdf"^^ . . . "Molecular and Cellular Analysis of the Nematostella vectensis Cnidom (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Molecular and Cellular Analysis of the Nematostella vectensis Cnidom (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Molecular and Cellular Analysis of the Nematostella vectensis Cnidom (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Molecular and Cellular Analysis of the Nematostella vectensis Cnidom (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Molecular and Cellular Analysis of the Nematostella vectensis Cnidom (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #12983 \n\nMolecular and Cellular Analysis of the Nematostella vectensis Cnidom\n\n" . "text/html" . . . "590 Tiere (Zoologie)"@de . "590 Zoological sciences"@en . .