<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Assembly and quality control of the V-ATPase in Arabidopsis"^^ . "The proteome of the model plant Arabidopsis thaliana is estimated to contain ~13000 non-redundant proteins (Baerenfaller et al., 2008). During their lifetime most of these proteins interact with each other, either via transient association or by assembling into stable protein complexes. These interactions are facilitated by chaperones and monitored by various quality control systems. This thesis presents data on both topics, using the plant vacuolar H+-ATPase (V-ATPase) as a model protein complex. In chapter 1 of this thesis the role of the ER localized proteins AtVMA12 and AtVMA22 are characterized in detail. Localization studies demonstrate that AtVMA22 is recruited to the ER membrane by AtVMA12. Artifical microRNAs directed against both assembly factors cause phenotypes characteristic for plants lacking V-ATPase activity, suggesting a pivotal role in V-ATPase assembly. Further biochemical characterization revealed a direct interaction between AtVMA12 and the integral membrane subunit VHA-a3 and an association of AtVMA22 with the peripheral subunit VHA-C of the V-ATPase. Taken together the results lead the author to postulate that AtVMA12 and AtVMA22 represent true assembly factors of the plant V-ATPase. In the second part of this work, the mechanism is characterized by which non-functional V-ATPase complexes are retained in the ER. Using a combination of pharmacological and genetic approaches it is demonstrated that V-ATPase activity and the membrane associated chaperone calnexin are required to retain non-functional protein complexes. However, non-functional complexes escaping the quality control negatively affect the fitness of the plant, underpinning the importance of a QC that retains non-functional proteins. "^^ . "2012" . . . . . . . . "Christoph"^^ . "Neubert"^^ . "Christoph Neubert"^^ . . . . . . "Assembly and quality control of the V-ATPase in Arabidopsis (PDF)"^^ . . . "complete_thesis_fina_01l.pdf"^^ . . . "Assembly and quality control of the V-ATPase in Arabidopsis (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Assembly and quality control of the V-ATPase in Arabidopsis (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Assembly and quality control of the V-ATPase in Arabidopsis (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Assembly and quality control of the V-ATPase in Arabidopsis (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Assembly and quality control of the V-ATPase in Arabidopsis (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #13463 \n\nAssembly and quality control of the V-ATPase in Arabidopsis\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .