<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Mechanisms of CD95-Clustering"^^ . "In this study I investigated the mechanisms of clustering of the apoptosis receptor CD95\r\nafter induction with its ligand and the oligomerization level before induction. Previous\r\nstudies demonstrated that receptor clustering is an essential part in apoptosis signaling.\r\nMostly using biochemical methods and nuclear magnetic resonance the essential role of\r\nvarious mechanisms in receptor clustering could be shown, such as lipid raft localization of\r\nthe receptor, clustering via the receptor death domain and internalization. An oligomerization\r\nof receptors before induction via a so called pre-ligand assembly domain could be\r\nshown at high concentrations, so that typically the receptor is assumed to be trimeric\r\nbefore induction. Moreover a consistent model of the role of all mechanisms in different\r\ncell types and direct observation on the plasma membrane is still missing. Therefore\r\nI investigated receptor clustering in single living cells on the cell membrane using microscopy.\r\nIn particular I quantified intensities to correlate with the number of molecules.\r\nand studied receptor clustering by dynamics measurements using fluorescence recovery\r\nafter photobleaching. I observed that receptor clustering is concentration dependent and\r\nin HeLa at high receptor concentrations the ligand as only crosslinking mechanism yields\r\nmean clusters size of seven molecules. Death domain assembly and lipid raft localization do not effect clustering in this cell type. At physiological expression levels the pre-ligand\r\nassembly shows an oligomer distribution with an eminent part of monomers or dimers.\r\nBy knockdown of the receptor the distribution can be pushed to monomers. I made a\r\nconsistent study for the type II cell line HeLa, considering many proposed clustering\r\nmechanisms and showed that ligand binding alone is a strong mechanism that can lead\r\nto high levels of clustering. I propose a model where the receptor is present as monomers\r\nor dimers at physiological concentrations in the absence of induction."^^ . "2012" . . . . . . . . "Johanna"^^ . "Berndt"^^ . "Johanna Berndt"^^ . . . . . . "Mechanisms of CD95-Clustering (PDF)"^^ . . . "Dissertation-JBerndt.pdf"^^ . . . "Mechanisms of CD95-Clustering (PDF)"^^ . . . "Summary-120911.pdf"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "small.jpg"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Mechanisms of CD95-Clustering (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #13904 \n\nMechanisms of CD95-Clustering\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .