eprintid: 1413
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/14/13
datestamp: 2001-01-24 00:00:00
lastmod: 2014-04-03 10:35:06
status_changed: 2012-08-14 15:00:54
type: doctoralThesis
metadata_visibility: show
creators_name: Kirsch, Wolfgang G.
title: Biophysical analysis of the spatial and temporal distribution of free Ca2+ ions within micro- and nanodomains of muscle cells
title_de: Biophysikalische Analyse der räumlichen und zeitlichen Verteilung freier Ca2+-Ionen in Mikro- und Nanodomänen von Muskelzellen
ispublished: pub
subjects: 610
divisions: 53200
adv_faculty: af-13
keywords: muscle , physiology , calcium , sparks , biophysics
cterms_swd: Muskelzelle
cterms_swd: Calciumkonzentration
cterms_swd: Calciumfreisetzung
abstract_translated_text: This work establishes methods to measure and quantify the spatial and temporal changes of the free Ca2+ ion concentration ([Ca2+]) inside skeletal muscle fibers and to calculate the underlying Ca2+ release from the sarcoplasmic reticulum (SR) caused by the opening of Ca2+ permeable ion channels (RyRs). The recording of microdomain Ca2+ signals (spatially averaged measurements of transient increases in myoplasmic [Ca2+]), was achieved by epi-illumination microscopy utilizing changes in the absorption of the Ca2+ indicator Antipyrylazo III. Nanodomain Ca2+ signals, the brief and highly localized spatio-temporal changes in [Ca2+] caused by elementary Ca2+ release events, were measured with submicron precision and a time resolution of 2 ms using confocal laser scanning fluorescence microscopy. The underlying release rate of Ca2+ ions from the SR was calculated using the most detailed information about intracellular Ca2+ buffers and Ca2+ pumps currently available. With these quantitative tools this work lead to the conclusion that the elementary events of Ca2+ release are generated by the opening of more than one RyR. Striking morphological differences between elementary Ca2+ release events measured under different experimental conditions are revealed, which can be explained by the molecular structure of the Ca2+ release source. For the first time this work presents measurements of Ca2+ sparks in adult mammalian muscle with high abundance which is of significant importance for medical diseases involving pathophysiological changes in Ca2+ regulation. 
abstract_translated_lang: eng
class_scheme: mesh
date: 2000
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00001413
ppn_swb: 1643183753
own_urn: urn:nbn:de:bsz:16-opus-14138
date_accepted: 2000-10-27
advisor: HASH(0x5561209c5a88)
language: eng
bibsort: KIRSCHWOLFBIOPHYSICA2000
full_text_status: public
citation:   Kirsch, Wolfgang G.  (2000) Biophysical analysis of the spatial and temporal distribution of free Ca2+ ions within micro- and nanodomains of muscle cells.  [Dissertation]     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/1413/1/Kirsch.pdf