TY - GEN A1 - Eberhard, Christian UR - https://archiv.ub.uni-heidelberg.de/volltextserver/14257/ CY - Heidelberg, Deutschland TI - Development of a Model System to Study Cell Adhesion and Cell Mechanics N2 - Within this work, a simplified model system was developed for the experimental investigation of physical mechanisms underlying cell adhesion. Cellular complexity was reduced to three essential components: the lipid bilayer, the cell adhesion molecule integrin and the cytoskeletal protein actin. Integrin was integrated in spherical lipid bilayers, called giant unilamellar vesicles (GUVs), which were additionally filled with actin. Integrin alphaIIb beta3 was purified from human platelet membranes with high yield. Initially, this integrin was reconstituted in small unilamellar vesicles (SUVs). By variation of lipid composition, vesicle size and detergent concentration, crucial parameters for efficient integration could be determined and incorporation efficiency was enhanced. Two different approaches were compared to grow GUVs: electroformation and spontaneous swelling on agarose-lipid films. Besides biochemical characterization, formation of GUVs was investigated by means of confocal laser scanning microscopy confirming successful integration of integrin into the bilayer of the giant vesicles. A novel method for the preparation of GUVs from purified platelet membranes was established. Platelet membranes were directly purified and grown to giant vesicles by electroformation. Interaction of these GUVs with nano-structured and bio-functionalized surfaces was investigated. Adhesion of these integrin-coated vesicles was characterized for the first time by reflection interference contrast microscopy (RICM), which allowed estimation of an average adhesion energy. KW - Modellsystem Riesenvesikel Integrin alphaIIb beta3 N1 - CD-ROM mit Movies im Druckexemplar enthalten unter der Signatur: 2013 U 1 AV - public ID - heidok14257 Y1 - 2012/12/11/ ER -