title: 4Pi Nanoscopy parallelized by line  scanning
creator: Curdt, Franziska
subject: 500
subject: 500 Natural sciences and mathematics
description: A superresolution scanning fluorescence microscope – or nanoscope – allows imaging structures smaller than the diffraction limit of light. The principal idea in targeted  readout microscopy methods is the on/off-switching of a subset of fluorophores inside the focal volume with the help of different reversible switchable optical fluorescence  transitions. If, in addition, the effective numerical aperture is doubled as in a 4Pi optical setup, the resolving power in axial direction of a far field microscope can be significantly increased. Due to the local field enhancement, a 4Pi augmented STimulated Emission Depletion (STED) setup exploits STED light in an effective  manner. To this date, it features the smallest effective scannning volume. However, the acquisition of the obtained image information with this slender volume requires smaller scanning steps and in consequence a longer overall acquisition process. This disadvantage can be compensated for by parallel readout and is relevant for a 4Pi-STED combination, in particular. The proposed image parallelization scheme allows to trade-off acquisition speed against required resolution. In this thesis, a 4Pi augmented line parallelized STED setup is presented that is, for the first time, able to resolve 20 nm fluorescent microspheres with a resolution of about 50 nm in two dimensions with a focal volume that is 10 times parallelized as compared to a single spot confocal volume.
date: 2013
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/14362/1/F_Curdt_Dissertation.pdf
identifier: DOI:10.11588/heidok.00014362
identifier: urn:nbn:de:bsz:16-heidok-143627
identifier:   Curdt, Franziska  (2013) 4Pi Nanoscopy parallelized by line scanning.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/14362/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng