TY  - GEN
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/14362/
N2  - A superresolution scanning fluorescence microscope ? or nanoscope ? allows imaging structures smaller than the diffraction limit of light. The principal idea in targeted
readout microscopy methods is the on/off-switching of a subset of fluorophores inside the focal volume with the help of different reversible switchable optical fluorescence
transitions. If, in addition, the effective numerical aperture is doubled as in a 4Pi optical setup, the resolving power in axial direction of a far field microscope can be significantly increased. Due to the local field enhancement, a 4Pi augmented STimulated Emission Depletion (STED) setup exploits STED light in an effective
manner. To this date, it features the smallest effective scannning volume. However, the acquisition of the obtained image information with this slender volume requires smaller scanning steps and in consequence a longer overall acquisition process. This disadvantage can be compensated for by parallel readout and is relevant for a 4Pi-STED combination, in particular. The proposed image parallelization scheme allows to trade-off acquisition speed against required resolution. In this thesis, a 4Pi augmented line parallelized STED setup is presented that is, for the first time, able to resolve 20 nm fluorescent microspheres with a resolution of about 50 nm in two dimensions with a focal volume that is 10 times parallelized as compared to a single spot confocal volume.
A1  - Curdt, Franziska
Y1  - 2013///
AV  - public
ID  - heidok14362
TI  - 4Pi Nanoscopy parallelized by line
scanning
ER  -