<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Quick and specific non-linear microscopy \r\nusing shaped pulses with durations down to 10fs"^^ . "Nonlinear optical microscopy is ideally suited for in vivo imaging: it provides label-free \r\ncontrast revealing intrinsic structural and chemical properties of the sample in a non-invasive \r\nway. Successful nonlinear microscopy relies on the use of pulsed lasers to obtain high signal \r\nlevels at moderate average laser power. In particular, broadband excitation increases the \r\nnonlinear generation efficiency as well as the spectral coverage. However, lower \r\nphotodamage thresholds and less straightforward signal interpretation have prevented its \r\napplication to sensitive samples.\r\nIn this thesis, a pulse shaper is used to tailor ultrashort pulses for optimal imaging. This work \r\nconcentrates on Coherent Anti-Stokes Raman Scattering (CARS) because it provides an \r\naccess to highly specific vibrational spectra. The main concept is to encode molecule-specific \r\ninformation directly in the excitation. This is realized either by direct tailoring in a shaperassisted variant of a Multiplex CARS setup or by phase shaping of a single ultrashort pulse \r\n(<10fs). The photon load reduction and the optimization of the pulse profile achieved by \r\nshaping are demonstrated with the imaging of polymer samples and sensitive biological \r\ntissue. The flexibility of the setup allows switching between spectrally resolved acquisition \r\nfor precise chemical mapping and single channel detection for rapid imaging. \r\nFurther nonlinear effects can likewise be controlled by pulse shaping. In this work, the \r\nsystematic modification of the relative intensities of Second Harmonic Generation (SHG), \r\nTwo-Photon Excited Fluorescence (TPEF) and CARS is investigated as well as selective \r\nexcitation of fluorophores and molecular vibrations. Multimodal imaging with shaped \r\nultrashort pulses proves to be particularly efficient for biological samples as illustrated by the \r\nimaging of plant cells and skin biopsies. "^^ . "2013" . . . . . . . "Jean"^^ . "Rehbinder"^^ . "Jean Rehbinder"^^ . . . . . . "Quick and specific non-linear microscopy \r\nusing shaped pulses with durations down to 10fs (PDF)"^^ . . . "Thesis_Final.pdf"^^ . . . "Quick and specific non-linear microscopy \r\nusing shaped pulses with durations down to 10fs (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Quick and specific non-linear microscopy \r\nusing shaped pulses with durations down to 10fs (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Quick and specific non-linear microscopy \r\nusing shaped pulses with durations down to 10fs (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Quick and specific non-linear microscopy \r\nusing shaped pulses with durations down to 10fs (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Quick and specific non-linear microscopy \r\nusing shaped pulses with durations down to 10fs (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #15254 \n\nQuick and specific non-linear microscopy \nusing shaped pulses with durations down to 10fs\n\n" . "text/html" . . . "530 Physik"@de . "530 Physics"@en . . . "540 Chemie"@de . "540 Chemistry and allied sciences"@en . .