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primary topic: How in vitro alterations in cellular energy pathways can overcome obstacles in drug research

How in vitro alterations in cellular energy pathways can overcome obstacles in drug research
type: Article
abstract: Autor: Swen Seeland Institut / Klinik: Institut für Molekular- und Zellbiologie der Hochschule Mannheim Doktorvater: Prof. Dr. Mathias Hafner The basic mission of the pharmaceutical industry is to understand disease and to bring safe and effective drugs to patients. Starting with the drug discovery process, for any particular disease a first step involves selecting a disease-specific target, then finding a suitable assay to determine the activity of molecules in relation to the selected target. This path is difficult and, lacking proper technology, is similar to the proverbial search for the needle in a haystack. However, once a molecule emerges as a successful candidate in the drug discovery process, it enters into drug development. The drug development process provides safety data prior to "first-in-man" trials. Drug discovery and development are extended, expensive, and inefficient processes with a success rate of only 0.01%. Placing these difficulties into perspective, it remains the desire of the pharmaceutical industry to develop novel, economical, reliable in vitro technologies to meet the above challenges. In this PhD thesis, a multiparametric cytosensor system was used for real-time identification of physiological parameters in living cells, and to meet the above-mentioned challenges in drug research. This technology allows us to simultaneously monitor extracellular acidification (pH changes), cellular respiration (oxygen consumption), and cellular morphology and adhesion (impedance measurements). All work done by a cell and all of the activities of life in general necessitate energy, commonly in the form of adenosine triphosphate (ATP). The regeneration of the consumed ATP leads to increased oxygen consumption and an excretion of acidifying side products (lactate and carbon dioxide), which can be monitored and used to deduce changes in physiological pathways. The first project of this thesis explored the influence of the P-glycoprotein (ABCB1) transporter …
date: 2013-10
author list: authors
type: EPrint
type: DoctoralThesisEPrint
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subject: 500 Naturwissenschaften und Mathematik
subject: 570 Biowissenschaften, Biologie

How in vitro alterations in cellular energy pathways can overcome obstacles in drug research (PDF)
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has file: Dissertation_Uni_HD_Swen_Seeland_(Oktober_2012).pdf
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Swen Seeland
type: Person
given name: Swen
family name: Seeland

How in vitro alterations in cellular energy pathways can overcome obstacles in drug research (Other)
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primary topic: How in vitro alterations in cellular energy pathways can overcome obstacles in drug research

500 Naturwissenschaften und Mathematik
type: Concept
preferred label: 500 Natural sciences and mathematics @en

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en