<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Characterization of Schizosachharomyces pombe Sup11p, a protein involved in beta-1,6-glucan biosynthesis"^^ . "Proteins that are extensively O-mannosylated constitute crucial components of the fungal cell wall. They are linked to cell wall polysaccharides forming a distinct mannan layer. Various mannoproteins are covalently attached to the cell wall beta-1,6-glucan via remnants of their glycosylphosphatidylinositol (GPI) -anchor.\r\nA multicopy-suppressor screen of a conditionally lethal Schizosaccharomyces pombe protein O-mannosyl transferase mutant (nmt81-oma2) identified sup11+. Sup11p shows significant homology to Saccharomyces cerevisiae Kre9 which is involved in beta-1,6-glucan synthesis although its exact function remains uncertain.\r\nThis study gives evidence that sup11+ is an essential gene that is required for beta-1,6-glucan formation. In a mutant with reduced sup11+ expression, beta-1,6-glucan was absent from the cell wall. Further, it is demonstrated that Sup11p is indispensable for proper septum assembly. A conditionally lethal nmt81-sup11 knock-down mutant shows severe morphological defects and malformation of the septum with massive accumulation of cell wall material at the centre of the closing septum. These depositions consist partially of beta-1,3-glucan which ought to be restricted to the primary septum. Analysis of the nmt81-sup11 mutant cell wall brought evidence that Gas2p, a member of the beta-1,3-glucanosyl-transferases GH72 family, plays a crucial role in accumulating the observed septum material depositions. Moreover, a transcriptome analysis performed on the nmt81-sup11 mutant identified significant regulation of several cell wall glucan modifying enzymes.\r\nMoreover, it is shown that Sup11p:HA is hypo-mannosylated when expressed in an O-mannosylation mutant background. The hypo-mannosylated Sup11p can be N-glycosylated on an unusual N-X-A sequon in the oma4D background. This unusual sequon is located inside a S/T-rich region which is prone to be highly O-mannosylated in wild type yeast, masking the N-X-A sequon for N-glycosylation. Competition between N- and O-glycosylation has been shown in S. cerevisiae, but this study presents the first example for S. pombe demonstrating that such competition is a general mechanism."^^ . "2014" . . . . . . . "Lisa"^^ . "Weber"^^ . "Lisa Weber"^^ . . . . . . "Characterization of Schizosachharomyces pombe Sup11p, a protein involved in beta-1,6-glucan biosynthesis (PDF)"^^ . . . "doktorarbeit final version.pdf"^^ . . . "Characterization of Schizosachharomyces pombe Sup11p, a protein involved in beta-1,6-glucan biosynthesis (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Characterization of Schizosachharomyces pombe Sup11p, a protein involved in beta-1,6-glucan biosynthesis (Other)"^^ . . . . . . "Characterization of Schizosachharomyces pombe Sup11p, a protein involved in beta-1,6-glucan biosynthesis (Other)"^^ . . . . . . "Characterization of Schizosachharomyces pombe Sup11p, a protein involved in beta-1,6-glucan biosynthesis (Other)"^^ . . . . . . "Characterization of Schizosachharomyces pombe Sup11p, a protein involved in beta-1,6-glucan biosynthesis (Other)"^^ . . . . . "HTML Summary of #15700 \n\nCharacterization of Schizosachharomyces pombe Sup11p, a protein involved in beta-1,6-glucan biosynthesis\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .