title: Fine-Structures, Lateral Correlation and Diffusion of Membrane-Associated Proteins on Biological Membrane Surfaces
creator: Abuillan, Wasim
subject: ddc-500
subject: 500 Natural sciences and mathematics
subject: ddc-530
subject: 530 Physics
description: The primary aim of this thesis is to investigate the lateral diffusion, correlation and interactions of proteins and peptides with cell membrane models by the combination of experimental techniques in real and reciprocal space. In Chapter 4, the characteristic distance and range of lateral correlation between non-crystalline proteins anchored to fluid lipid monolayers at high surface densities were determined by grazing incidence small angle X-ray scattering (GISAXS) for the first time. Moreover, the lateral density of membrane-anchored proteins could be quantified from Sulfur Kα emission detected by grazing incidence X-ray fluorescence (GIXF). In Chapter 5, the influence of molecular crowding on the lateral diffusion of membrane-anchored proteins was investigated by fluorescence recovery after photobleaching and single particle tracking microscopy, yielding a clear transition from free diffusion to confined diffusion. In Chapter 6, the interactions between antimicrobial peptides and bacterial membrane models are probed by GIXF that allows for the identification of the spatial localization of ions with Ångstrom accuracy. This enabled one to discriminate different "modes" of membrane-protein interactions, such as adsorption and incorporation on the molecular level. The obtained results demonstrated that the use of real and reciprocal space techniques can provide information about fine-structures, electrostatics, and dynamic correlation at biological interfaces.
date: 2013
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/15735/1/Thesis_Wasim%20Abuillan%202013.pdf
identifier: DOI:10.11588/heidok.00015735
identifier: urn:nbn:de:bsz:16-heidok-157351
identifier:   Abuillan, Wasim  (2013) Fine-Structures, Lateral Correlation and Diffusion of Membrane-Associated Proteins on Biological Membrane Surfaces.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/15735/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng