…
comment: The repository administrator has not yet configured an RDF license.
primary topic: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients

An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients
type: Article
abstract: Human African Trypanosomiasis (HAT) is a neglected tropical disease that mainly affects the poorest people in sub Saharan Africa. HAT is caused by two subspecies of Trypanosoma brucei; T. b. rhodesiense is found in Eastern Africa and causes the acute form of the disease, while T. b. gambiense is found in Western Africa and causes the chronic form of the disease. With no reliable diagnostic screening test, available drugs being rather toxic, and emerging cases of drug resistant strains, research on the molecular aspects of the trypanosome is being carried out with the hope of identifying potential drug targets and diagnostic markers. Since most studies are carried out on cultured blood stream trypanosomes, the extent to which these parasites are representative of a real human infection is not known. Therefore the aim of this study was to analyze the transcriptome of clinical isolates of T. b. rhodesiense from patient peripheral blood and cerebral spinal fluid by high throughput sequencing. But given the low parasitaemia during active infection, I developed a splice leader priming based Polymerase chain reaction method to specifically amplify nanogram amounts of trypanosome total RNA in microgram amounts of Human cellular RNA, to an amount sufficient for sequencing. The amplification method resulted in trypanosome transcripts covering 60% of the T. brucei 6772 unique genes, and with an expression threshold of 5 Rpkm. The sequenced amplified libraries (four replicates) were highly reproducible and comparable to unamplified libraries generated in the same way. However a comparison to the conventional RNASeq generated library showed distortions in the transcriptome, which could be corrected for and used to analyze clinical samples. An analysis of methods used to purify trypanosomes from blood showed that; even though DEAE chromatography and reticulocyte lysis resulted in 10 times more parasites than those in the buffy coat isolation method, reticulocyte lysis resulted in…
date: 2013
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
has document: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (Other)
subject: 570 Biowissenschaften, Biologie

An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
type: Document
has file: Thesis.pdf
has part: Thesis.pdf

Julius Mulindwa
type: Person
given name: Julius
family name: Mulindwa

An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (Other)
type: Document
is version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
is volatile version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
is index codes version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
has file: indexcodes.txt
has part: indexcodes.txt

An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (Other)
type: Document
is version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
is volatile version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
islightbox thumbnail version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
has file: lightbox.jpg
has part: lightbox.jpg

An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (Other)
type: Document
is version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
is volatile version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
ispreview thumbnail version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
has file: preview.jpg
has part: preview.jpg

An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (Other)
type: Document
is version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
is volatile version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
ismedium thumbnail version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
has file: medium.jpg
has part: medium.jpg

An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (Other)
type: Document
is version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
is volatile version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
issmall thumbnail version of: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients (PDF)
has file: small.jpg
has part: small.jpg

HTML Summary of #15863 An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients
format: text/html
primary topic: An approach to determine the Transcriptome of T. b. rhodesiense from Sleeping Sickness patients

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en