<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Localization of Subcellular Structures with\r\nSuper Resolution Light Microscopy on Thin\r\nSections of Nervous Tissue"^^ . "Release of synaptic vesicles (SV) is a process that is orchestrated by proteins present in the presynaptic terminus called the active zone (AZ). Knowledge of the\r\nplacement of proteins is necessary to understand how SV release occurs. There is limited information on the location of the AZ proteins from studies of biochemical\r\nassays or immuno-electron microscope.\r\nDevelopments in fluorescence light microscopy are capable of reaching subnanometer resolution and therefore can be used to image multiple proteins of the AZ.\r\nFor instance, a technique called direct Stochastic Optical Reconstruction Microscopy (dSTORM) can reach a resolution of 20 nm in the x-y plane, which is an order of\r\nmagnitude greater than conventional light microscope. This work is devoted to developing techniques, which enables the use of dSTORM on thick brain tissue samples. In this respect, two thick tissue handling techniques have been explored, namely tomoSTORM and Tokuyasu’s ultracryotomy. Using tomoSTORM, we could construct a super-resolution 3D structure of the calyx of Held synapse. In addition, we\r\nalso demonstrate multicolor capability by being able to localize the abundantly distributed mitochondria to the synaptic compartment of the calyx of Held. Due to\r\nantibody staining limitations, Tokuyasu’s ultracryotomy was explored. Using this approach we gathered dual-color super-resolution data in the calyx of Held on the\r\ndistribution of Bassoon with respect to Piccolo. In agreement with the standardresolution\r\nmicroscopy, overview image of Bassoon and Piccolo show that both\r\nproteins exist together in the majority of the AZs. In addition we can show at the\r\nnanoscopic level in a given AZ that the two proteins not only exist as separate entities\r\nbut are also found to be colocalized. We also gathered data on the distribution of\r\nSeptin 5 and Piccolo and found that at P7 Septin 5 and Piccolo colocalize while at\r\nP17 they do not colocalize. This observation is consistent with the finding that Septin\r\n5 may cluster voltage gated calcium channels at P7 at the AZ.\r\nIn addition, as dSTORM is limited to photoswitching of 2 dyes, efforts were\r\nmade to extend this. To this extent, we show efficient photoswitching of phalloidin\r\nconjugated to ATTO 488, TRITC and BODIPY 650.\r\n8\r\nIn summary, this thesis is focused on adapting dSTORM to thick tissue\r\nsamples and developing multicolor photoswitching probes to explore multiple protein\r\ndistribution in the synaptic compartments of mammalian brain tissue."^^ . "2014" . . . . . . . "Siddharth "^^ . "Nanguneri"^^ . "Siddharth Nanguneri"^^ . . . . . . "Localization of Subcellular Structures with\r\nSuper Resolution Light Microscopy on Thin\r\nSections of Nervous Tissue (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Localization of Subcellular Structures with\r\nSuper Resolution Light Microscopy on Thin\r\nSections of Nervous Tissue (PDF)"^^ . . . "Doctoral thesis_Siddharth Nanguneri.pdf"^^ . . . "Localization of Subcellular Structures with\r\nSuper Resolution Light Microscopy on Thin\r\nSections of Nervous Tissue (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Localization of Subcellular Structures with\r\nSuper Resolution Light Microscopy on Thin\r\nSections of Nervous Tissue (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Localization of Subcellular Structures with\r\nSuper Resolution Light Microscopy on Thin\r\nSections of Nervous Tissue (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Localization of Subcellular Structures with\r\nSuper Resolution Light Microscopy on Thin\r\nSections of Nervous Tissue (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #17783 \n\nLocalization of Subcellular Structures with \nSuper Resolution Light Microscopy on Thin \nSections of Nervous Tissue\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . .