eprintid: 17815
rev_number: 27
eprint_status: archive
userid: 1329
dir: disk0/00/01/78/15
datestamp: 2014-12-16 13:29:34
lastmod: 2016-01-11 08:08:57
status_changed: 2014-12-16 13:29:34
type: article
metadata_visibility: show
creators_name: Kasten, Philipp
creators_name: Beverungen, Mirjam
creators_name: Lorenz, Helga
creators_name: Wieland, Julia
creators_name: Fehr, Michael
creators_name: Geiger, Florian
title: Comparison of platelet-rich plasma and VEGF-transfected mesenchymal stem cells on vascularization and bone formation in a critical-size bone defect
subjects: ddc-610
divisions: i-911460
abstract: Both platelet-rich plasma (PRP) and vascular endothelial growth factor (VEGF) can promote regeneration. The aim of this study was to compare the effects of these two elements on bone formation and vascularization in combination with bone marrow stromal cells (BMSC) in a critical-size bone defect in rabbits. The critical-size defects of the radius were filled with: (1) a calcium-deficient hydroxyapatite (CDHA) scaffold + phVEGF(165)-transfected BMSC (VEGF group), (2) CDHA and PRP, or (3) CDHA, autogenous BMSC, and PRP. As controls served: (4) the CDHA scaffold alone and (5) the CDHA scaffold and autogenous BMSC. The volume of new bone was measured by means of micro-CT scans, and vascularization was assessed in histology after 16 weeks. Bone formation was higher in the PRP + CDHA, BMSC + CDHA, and PRP + BMSC + CDHA groups than in the VEGF group (p < 0.05). VEGF transfection significantly promoted vascularization of the scaffolds in contrast to BMSC and PRP (p < 0.05), but was similar to the result of the CDHA + PRP + BMSC group. The results show that VEGF-transfected BMSC as well as the combination of PRP and BMSC improve vascularization, but bone healing was better with the combination of BMSC and PRP than with VEGF-transfected BMSC. Expression of VEGF in BMSC as a single growth factor does not seem to be as effective for bone formation as expanded BMSC alone or PRP which contains a mixture of growth factors. Copyright (C) 2012 S. Karger AG, Basel
date: 2012
publisher: S. Karger AG
id_scheme: DOI
id_number: 10.11588/heidok.00017815
ppn_swb: 1653748494
own_urn: urn:nbn:de:bsz:16-heidok-178159
language: eng
bibsort: KASTENPHILCOMPARISON2012
full_text_status: public
publication: Cells tissues organs
volume: 196
number: 6
pagerange: 523-533
issn: 1422-6405
citation:   Kasten, Philipp ; Beverungen, Mirjam ; Lorenz, Helga ; Wieland, Julia ; Fehr, Michael ; Geiger, Florian  (2012) Comparison of platelet-rich plasma and VEGF-transfected mesenchymal stem cells on vascularization and bone formation in a critical-size bone defect.  Cells tissues organs, 196 (6).  pp. 523-533.  ISSN 1422-6405     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/17815/1/CTO2012196006523.pdf