title: Challenges and pitfalls of experimental bariatric procedures in rats
creator: Fischer, Lars
creator: Clemens, Gabriella
creator: Gehrig, Tobias
creator: Kenngott, Hannes
creator: Becker, Kristianna
creator: Bruckner, Thomas
creator: Gutt, Carsten N.
creator: Büchler , Markus W.
creator: Müller-Stich, Beat
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Introduction: The impact of Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) on obesity and obesity-related diseases is unquestionable. Up to now, the technical descriptions of these techniques in animals/rats have not been very comprehensive. Methods: For SG and RYGB, operating time, learning curve, and intraoperative mortality in relation to weight of the rat and type of anesthesia were recorded. Furthermore, a review of the literature on experimental approaches towards SG and RYGB in rats was carried out, merging in a detailed technical description for both procedures. Results: The data presented here revealed that the mean operating time for SG (69.4 +/- 22.2 min (SD)) was shorter than for RYGB (123.0 +/- 20.7 min). There is a learning curve for both procedures, resulting in a reduced operating time of up to 60% in SG and 35% in RYGB (p < 0.05; t-test). However, with increased weight, operating time increases to about 80 min for SG and about 120 min for RYGB. Obese rats have an increased intraoperative mortality rate of up to 50%. After gaseous anesthesia the mortality can be even higher. The literature search revealed 40 papers dealing with SG and RYGB in rats. 18 articles (45%) contained neither photographs nor illustrations; 14 articles (35%) did not mention the applied type of anesthesia. The mortality rate was described in 15 papers (37.5%). Conclusion: Experimental obesity surgery in rats is challenging. Because of the high mortality in obese rats operated under gaseous anesthesia, exercises to establish the techniques should be performed in small rats using intraperitoneal anesthesia. Copyright (C) 2012 S. Karger GmbH, Freiburg
publisher: S. Karger AG
date: 2012
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/17826/1/OFA2012005003359.pdf
identifier: DOI:10.11588/heidok.00017826
identifier: urn:nbn:de:bsz:16-heidok-178264
identifier:   Fischer, Lars ; Clemens, Gabriella ; Gehrig, Tobias ; Kenngott, Hannes ; Becker, Kristianna ; Bruckner, Thomas ; Gutt, Carsten N. ; Büchler , Markus W. ; Müller-Stich, Beat  (2012) Challenges and pitfalls of experimental bariatric procedures in rats.  Obesity facts, 5 (3).  pp. 359-371.  ISSN 1662-4025     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/17826/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng