<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Funktionelle Charakterisierung des putativen Tumorsuppressors \"Epithelial Membrane Protein 3\""^^ . "EMP3 has been proposed as a potential tumor suppressor gene in neuroblastomas,\r\ngliomas and other solid tumors, due to its differential methylation and expression pattern.\r\nIn these tumors EMP3 is often transcriptionally silenced by promoter hypermethylation.\r\nThe biological function of EMP3 itself is largely unknown. Based on homologies to other\r\nmembers of the protein family, it was presumed that EMP3 is involved in the regulation of\r\nproliferation, apoptosis and cell-cell-interactions.\r\nThe aim of this work is the functional characterization of EMP3 and its role in glioma\r\nformation and progression. To this end we analyzed the methylation and expression\r\npattern of EMP3 in gliomas, non-neoplastic tissues and cell lines. In addition we studied\r\nthe effects of RNA-interference-mediated knockdown of EMP3 on proliferation, migration\r\nand apoptosis in an in vitro cancer cell line model. Furthermore we utilized different\r\ninteraction assays to identify novel protein-protein interaction partners of EMP3.\r\nEMP3 is expressed in almost all analyzed normal tissues, with the highest levels in\r\nleukocytes, neurons and astrocytic cells. In gliomas, the EMP3 protein levels are highest in\r\nglioblastomas, of which over 80% show very strong EMP3 expression, while in low-grade\r\ngliomas only 20% show elevated levels of EMP3. High levels of EMP3 also correlate with\r\nshorter progression-free and overall patient survival. RNA-interference-mediated\r\nrepression of EMP3 significantly reduces the proliferation and migration of cancer cells in\r\nvitro and increased their susceptibility to induced cell death. This is in part caused by\r\ndecreased phosphorylation and activation of the EGFR, AKT and ERK signaling kinases.\r\nEMP3 is part of a complex interaction and signaling network, of which we could identify 10\r\nnovel interacting proteins. Through this network EMP3 is possibly involved in the\r\nregulation of several important signaling and trafficking pathways. EMP3 is therefore likely\r\nto be a mediator and regulator of intracellular trafficking and signal transduction.\r\nThe data support a role for EMP3 in the progression of cancer, but, at least in glioma, not\r\nas a tumor suppressor. Nevertheless EMP3 could be a valid prognostic and possibly even\r\npredictive biomarker in the diagnosis of glioma as well as a potential novel therapeutic\r\ntarget in different tumors and other diseases."^^ . "2014" . . . . . . . "Arne"^^ . "Christians"^^ . "Arne Christians"^^ . . . . . . "Funktionelle Charakterisierung des putativen Tumorsuppressors \"Epithelial Membrane Protein 3\" (PDF)"^^ . . . "Christians, A., Funktionelle Charakterisierung von EMP3. Dissertation (Finale Version).pdf"^^ . . . "Funktionelle Charakterisierung des putativen Tumorsuppressors \"Epithelial Membrane Protein 3\" (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Funktionelle Charakterisierung des putativen Tumorsuppressors \"Epithelial Membrane Protein 3\" (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Funktionelle Charakterisierung des putativen Tumorsuppressors \"Epithelial Membrane Protein 3\" (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Funktionelle Charakterisierung des putativen Tumorsuppressors \"Epithelial Membrane Protein 3\" (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Funktionelle Charakterisierung des putativen Tumorsuppressors \"Epithelial Membrane Protein 3\" (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #17866 \n\nFunktionelle Charakterisierung des putativen Tumorsuppressors \"Epithelial Membrane Protein 3\"\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . .