TY  - JOUR
Y1  - 2014///
ID  - heidok18655
AV  - public
VL  - 9
N2  - Bupleurum marginatum Wall. ex DC (Apiaceae) is a perennial herb widely used in traditional Chinese and Kampo medicine for the treatment of various infectious diseases. The biological activities of B. marginatum have not been fully investigated. This study aims to investigate the antitrypanosomal, antimicrobial and antiviral activities of methanol (ME) and dichloromethane (DCM) extracts of B. marginatum aerial parts and the ability of both extracts to inhibit the growth of different cancer cell lines.   Methods Phytochemical characterization of the extracts was performed by LC-MS profiling. The antitrypanosomal activity was evaluated using the resazurin method. The antimicrobial activity was assessed using agar diffusion and microdilution methods, and the minimum inhibitory concentration (MIC) values were determined. The antiviral activity was determined for 6.25, 12.5, and 50 ?g/mL doses using a plaque reduction assay. Cytotoxicity was investigated in eight cancer cell lines (Caco-2, CCL-81, CCRF-CEM, COS-7, HL-60, MIA PaCa-2, MCF-7, and PANC-1) using the MTT assay and the caspase 3/7 activity was determined over the range of 62.5?1000 ?g/mL.   Results Phytochemical analyses resulted in the characterization of 15 components, mainly flavonoids and lignans. The DCM extract showed significant antitrypanosomal activity (IC50: 36.21 ?g/mL) and moderate activity against Streptococcus pyogenes (MIC value: 0.25 mg/mL). At a dose of 12.5 ?g/mL, the DCM extract inhibited 73.6% of the plaque production by hepatitis A virus. CCRF-CEM cells were the most sensitive to both extracts (IC50: 12.5?22.7 ?g/mL). The cytotoxicity was mediated by induction of apoptosis (19-fold increase in the cellular caspase 3/7 level after treatment with the DCM extract at 1 mg/mL).   Conclusions ME and DCM extract of B. marginatum showed anti-infective and antiproliferative effects.
SP  - 1
CY  - London
EP  - 10
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/18655/
SN  - 1749-8546
PB  - BioMed Central
JF  - Chinese Medicine
TI  - Anti-infective and cytotoxic properties of Bupleurum marginatum
IS  - 4
A1  - Ashour, Mohamed L
A1  - Readi, Mahmoud Z
A1  - Hamoud, Razan
A1  - Eid, Safaa Y
A1  - Ahmady, Sherweit H
A1  - Nibret, Endalkachew
A1  - Herrmann, Florian
A1  - Youns, Mahmoud
A1  - Tahrani, Ahmed
A1  - Kaufmann, Dorothea
A1  - Wink, Michael
ER  -