eprintid: 19346
rev_number: 16
eprint_status: archive
userid: 1589
dir: disk0/00/01/93/46
datestamp: 2015-11-24 14:31:42
lastmod: 2024-04-03 10:47:58
status_changed: 2015-11-24 14:31:42
type: article
metadata_visibility: show
creators_name: Veres, Gábor
creators_name: Radovits, Tamás
creators_name: Merkely, Béla
creators_name: Karck, Matthias
creators_name: Szabó, Gábor
title: Custodiol-N, the novel cardioplegic solution reduces ischemia/reperfusion injury after cardiopulmonary bypass
subjects: ddc-610
divisions: i-910200
abstract: Backgrounds: On the basis of Custodiol preservation and cardioplegic solution a novel cardioplegic solution was developed to improve the postischemic cardiac and endothelial function. In this study, we investigated whether its reduced cytotoxicity and its ability to reduce reactive oxygen species generation during hypoxic condition have beneficial effects in a clinically relevant canine model of CPB.   Methods: 12 dogs underwent cardiopulmonary bypass with 60 minutes of hypothermic cardiac arrest. Dogs were divided into 2 groups: Custodiol (n = 6) and Custodiol-N (n = 6) (addition of L-arginin, N-α-acetyl-L-histidine and iron-chelators: deferoxamine and LK-614). Left ventricular hemodynamic variables were measured by a combined pressure-volume conductance catheter at baseline and after 60 minutes of reperfusion. Coronary blood flow, myocardial ATP content, plasma nitrate/nitrite and plasma myeloperoxidase levels were also determined.   Results: The use of Custodiol-N cardioplegic solution improved coronary blood flow (58 ± 7 ml/min vs. 26 ± 3 ml/min) and effectively prevented cardiac dysfunction after cardiac arrest. In addition, the myocardial ATP content (12,8 ± 1,0 μmol/g dry weight vs. 9,5 ± 1,5 μmol/g dry weight) and plasma nitrite (1,1 ± 0,3 ng/ml vs. 0,5 ± 0,2 ng/ml) were significantly higher after application of the new cardioplegic solution. Furthermore, plasma myeloperoxidase level (3,4 ± 0,4 ng/ml vs. 4,3 ± 2,2 ng/ml) significantly decreased in Custodiol-N group.   Conclusions: The new HTK cardioplegic solution (Custodiol-N) improved myocardial and endothelial function after cardiopulmonary bypass with hypothermic cardiac arrest. The observed protective effects imply that the Custodiol-N could be the next generation cardioplegic solution in the protection against ischemia-reperfusion injury in cardiac surgery.  
date: 2015
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1653549270
own_urn: urn:nbn:de:bsz:16-heidok-193463
language: eng
bibsort: VERESGABORCUSTODIOLN2015
full_text_status: public
publication: Journal of cardiothoracic surgery
volume: 10
number: 27
place_of_pub: London
pagerange: 1-7
issn: 1749-8090
citation:   Veres, Gábor ; Radovits, Tamás ; Merkely, Béla ; Karck, Matthias ; Szabó, Gábor  (2015) Custodiol-N, the novel cardioplegic solution reduces ischemia/reperfusion injury after cardiopulmonary bypass.  Journal of cardiothoracic surgery, 10 (27).  pp. 1-7.  ISSN 1749-8090     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19346/1/13019_2015_Article_226.pdf