<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique"^^ . "Background: According to current clinical guidelines mutational analysis for KRAS and NRAS is recommended prior to EGFR-directed therapy of colorectal cancer (CRC) in the metastatic setting. Therefore, reliable, fast, sensitive and cost-effective methods for routine tissue based molecular diagnostics are required that allow the assessment of the CRC mutational status in a high throughput fashion. Methods: We have developed a custom designed assay for routine mass-spectrometric (MS) (MassARRAY®, Agena Bioscience) analysis to test the presence/absence of 18 KRAS, 14 NRAS and 4 BRAF mutations. We have applied this assay to 93 samples from patients with CRC and have compared the results with Sanger sequencing and a chip hybridization assay (KRAS LCD-array Kit, Chipron). In cases with discordant results, next-generation sequencing (NGS) was performed. Results: MS detected a KRAS mutation in 46/93 (49 %), a NRAS mutation in 2/93 (2 %) and a BRAF mutation in 1/93 (1 %) of the cases. MS results were in agreement with results obtained by combination of the two other methods in 92 (99 %) of 93 cases. In 1/93 (1 %) of the cases a G12V mutation has been detected by Sanger sequencing and MS, but not by the chip assay. In this case, NGS has confirmed the G12V mutation in KRAS. Conclusions: Mutational analysis by MS is a reliable method for routine diagnostic use, which can be easily extended for testing of additional mutations. "^^ . "2015" . . "10" . "132" . . "BioMed Central"^^ . . . "Diagnostic pathology"^^ . . . "17461596" . . . . . . . . . . . . . . . . . "Jörg"^^ . "Kriegsmann"^^ . "Jörg Kriegsmann"^^ . . "Norbert"^^ . "Arens"^^ . "Norbert Arens"^^ . . "Volker"^^ . "Endris"^^ . "Volker Endris"^^ . . "Mark"^^ . "Kriegsmann"^^ . "Mark Kriegsmann"^^ . . "Wilko"^^ . "Weichert"^^ . "Wilko Weichert"^^ . . . . . . "Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique (PDF)"^^ . . . "13000_2015_Article_364.pdf"^^ . . . "Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique (Other)"^^ . . . . . . "small.jpg"^^ . . . "Detection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #19380 \n\nDetection of KRAS, NRAS and BRAF by mass spectrometry - a sensitive, reliable, fast and cost-effective technique\n\n" . "text/html" . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .