title: p16INK4a/Ki-67 dual stain cytology for cervical cancer screening in Thika district, Kenya
creator: Wangari Ngugi, Caroline
creator: Schmidt, Dietmar
creator: Wanyoro, Karanja
creator: Boga, Hamadi
creator: Wanzala, Peter
creator: Muigai, Anne
creator: Mbithi, John
creator: von Knebel-Doeberitz, Magnus
creator: Reuschenbach, Miriam
subject: ddc-570
subject: 570 Life sciences
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Background: The identification of suited early detection tests is one among the multiple requirements to reduce cervical cancer incidence in developing countries.   Methods: We evaluated p16INK4a/Ki-67 dual-stain cytology in a screening population in Thika district, Kenya and compared it to high-risk human papillomavirus (HR-HPV) DNA testing and visual inspection by acetic acid (VIA) and Lugol’s iodine (VILI).   Results: Valid results for all tests could be obtained in 477 women. 20.9 % (100/477) were tested positive for HR-HPV DNA, 3.1 % (15/477) had positive VIA/VILI and 8.2 % (39/477) positive p16INK4a/Ki-67 cytology. Of 22 women that showed up for colposcopy and biopsy, 6 women were diagnosed with CIN3 and two with CIN2. All women with CIN2/3 were negative in VIA/VILI screening and positive by HR-HPV DNA testing. But HPV was also positive in 91.7 % (11/12) of women with normal histology. p16INK4a/Ki-67 cytology was positive in all 6 women with CIN3, in one of the two CIN2 and in only 8.3 % (1/12) of women with normal histology.   Conclusions: p16INK4a/Ki-67 cytology is an interesting test for further studies in developing countries, since our findings point to a lower fraction of false positive test results using p16INK4a/Ki-67 cytology compared to HPV DNA testing in a Kenyan screening population. VIA/VILI missed all histology-proven CIN2/3.  
publisher: BioMed Central
date: 2015
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/19386/1/13027_2015_Article_20.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-193864
identifier:   Wangari Ngugi, Caroline ; Schmidt, Dietmar ; Wanyoro, Karanja ; Boga, Hamadi ; Wanzala, Peter ; Muigai, Anne ; Mbithi, John ; von Knebel-Doeberitz, Magnus ; Reuschenbach, Miriam  (2015) p16INK4a/Ki-67 dual stain cytology for cervical cancer screening in Thika district, Kenya.  Infectious agents and cancer, 10 (25).  pp. 1-6.  ISSN 1750-9378     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/19386/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng