eprintid: 19386
rev_number: 36
eprint_status: archive
userid: 1589
dir: disk0/00/01/93/86
datestamp: 2015-12-14 12:29:04
lastmod: 2024-04-10 18:07:12
status_changed: 2015-12-14 12:29:04
type: article
metadata_visibility: show
creators_name: Wangari Ngugi, Caroline
creators_name: Schmidt, Dietmar
creators_name: Wanyoro, Karanja
creators_name: Boga, Hamadi
creators_name: Wanzala, Peter
creators_name: Muigai, Anne
creators_name: Mbithi, John
creators_name: von Knebel-Doeberitz, Magnus
creators_name: Reuschenbach, Miriam
title: p16INK4a/Ki-67 dual stain cytology for cervical cancer screening in Thika district, Kenya
subjects: ddc-570
subjects: ddc-610
divisions: i-912000
abstract: Background: The identification of suited early detection tests is one among the multiple requirements to reduce cervical cancer incidence in developing countries.   Methods: We evaluated p16INK4a/Ki-67 dual-stain cytology in a screening population in Thika district, Kenya and compared it to high-risk human papillomavirus (HR-HPV) DNA testing and visual inspection by acetic acid (VIA) and Lugol’s iodine (VILI).   Results: Valid results for all tests could be obtained in 477 women. 20.9 % (100/477) were tested positive for HR-HPV DNA, 3.1 % (15/477) had positive VIA/VILI and 8.2 % (39/477) positive p16INK4a/Ki-67 cytology. Of 22 women that showed up for colposcopy and biopsy, 6 women were diagnosed with CIN3 and two with CIN2. All women with CIN2/3 were negative in VIA/VILI screening and positive by HR-HPV DNA testing. But HPV was also positive in 91.7 % (11/12) of women with normal histology. p16INK4a/Ki-67 cytology was positive in all 6 women with CIN3, in one of the two CIN2 and in only 8.3 % (1/12) of women with normal histology.   Conclusions: p16INK4a/Ki-67 cytology is an interesting test for further studies in developing countries, since our findings point to a lower fraction of false positive test results using p16INK4a/Ki-67 cytology compared to HPV DNA testing in a Kenyan screening population. VIA/VILI missed all histology-proven CIN2/3.  
date: 2015
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1656056224
own_urn: urn:nbn:de:bsz:16-heidok-193864
language: eng
bibsort: WANGARINGUP16INK4AKI2015
full_text_status: public
publication: Infectious agents and cancer
volume: 10
number: 25
place_of_pub: London
pagerange: 1-6
issn: 1750-9378
citation:   Wangari Ngugi, Caroline ; Schmidt, Dietmar ; Wanyoro, Karanja ; Boga, Hamadi ; Wanzala, Peter ; Muigai, Anne ; Mbithi, John ; von Knebel-Doeberitz, Magnus ; Reuschenbach, Miriam  (2015) p16INK4a/Ki-67 dual stain cytology for cervical cancer screening in Thika district, Kenya.  Infectious agents and cancer, 10 (25).  pp. 1-6.  ISSN 1750-9378     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19386/1/13027_2015_Article_20.pdf