eprintid: 19430
rev_number: 14
eprint_status: archive
userid: 1589
dir: disk0/00/01/94/30
datestamp: 2016-01-08 13:33:01
lastmod: 2024-04-10 07:29:27
status_changed: 2016-01-08 13:33:01
type: article
metadata_visibility: show
creators_name: Schneeweiß, Andreas
creators_name: Chia, Stephen
creators_name: Hegg, Roberto
creators_name: Tausch, Christoph
creators_name: Deb, Rahul
creators_name: Ratnayake, Jayantha
creators_name: McNally, Virginia
creators_name: Ross, Graham
creators_name: Kiermaier, Astrid
creators_name: Cortés, Javier
title: Evaluating the predictive value of biomarkers for efficacy outcomes in response to pertuzumab- and trastuzumab-based therapy: an exploratory analysis of the TRYPHAENA study
subjects: ddc-610
divisions: i-910400
abstract: Introduction: Molecular markers that predict responses to particular therapies are invaluable for optimization of patient treatment. The TRYPHAENA study showed that pertuzumab and trastuzumab with chemotherapy was an efficacious and tolerable combination for patients with human epidermal growth factor receptor 2 (HER2)-positive breast cancer in the neoadjuvant setting. We analyzed whether particular biomarkers correlated with the responses observed and therefore may predict outcomes in patients given pertuzumab plus trastuzumab.   Methods: We describe the analysis of a panel of biomarkers including HER2, human epidermal growth factor receptor 3 (HER3), epidermal growth factor receptor (EGFR), phosphatase and tensin homolog (PTEN), and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) by qRT-PCR, immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), enzyme-linked immunosorbent assay (ELISA), and PCR-based mutational analyses as appropriate. For each marker analyzed, patients were categorized into ‘low’ (generally below median) or ‘high’ (generally above median) subgroups at baseline and post-treatment.   Results: Correlation of marker subgroups with the achievement of a pathological complete response (pCR) (ypT0/is) was analyzed. HER2 protein and mRNA expression levels were associated with pCR rate in two of the three study arms and the pooled analyses. Correlations of biomarker status with pCR occurred in one individual arm only and the pooled analyses with EGFR and PTEN; however, interpretation of these results is limited by a strong imbalance in patient numbers between the high and low subgroups and inconsistency between arms. We also found no association between expression levels of TOP2A and pCR rate in either the anthracycline-containing or free arms of TRYPHAENA.   Conclusions: According to these analyses, and in line with other analyses of pertuzumab and trastuzumab in the neoadjuvant setting, we conclude that HER2 expression remains the only marker suitable for patient selection for this regimen at present.   Trial registration: The TRYPHAENA study was registered with ClinicalTrials.gov, NCT00976989, on September 14 2009.  
date: 2014
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1656777266
own_urn: urn:nbn:de:bsz:16-heidok-194302
language: eng
bibsort: SCHNEEWEISEVALUATING2014
full_text_status: public
publication: Breast Cancer Research
volume: 16
number: R73
place_of_pub: London
pagerange: 1-12
issn: 1465-542X
citation:   Schneeweiß, Andreas ; Chia, Stephen ; Hegg, Roberto ; Tausch, Christoph ; Deb, Rahul ; Ratnayake, Jayantha ; McNally, Virginia ; Ross, Graham ; Kiermaier, Astrid ; Cortés, Javier  (2014) Evaluating the predictive value of biomarkers for efficacy outcomes in response to pertuzumab- and trastuzumab-based therapy: an exploratory analysis of the TRYPHAENA study.  Breast Cancer Research, 16 (R73).  pp. 1-12.  ISSN 1465-542X     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19430/1/13058_2013_Article_3562.pdf