eprintid: 19435
rev_number: 17
eprint_status: archive
userid: 1589
dir: disk0/00/01/94/35
datestamp: 2015-12-22 10:26:56
lastmod: 2024-05-08 19:08:21
status_changed: 2015-12-22 10:26:56
type: article
metadata_visibility: show
creators_name: Sancar, Cigdem
creators_name: Sancar, Gencer
creators_name: Ha, Nati
creators_name: Cesbron, François
creators_name: Brunner, Michael
title: Dawn- and dusk-phased circadian transcription rhythms coordinate anabolic and catabolic functions in Neurospora 
subjects: ddc-540
subjects: ddc-570
divisions: i-711000
abstract: Background: Circadian clocks control rhythmic expression of a large number of genes in coordination with the 24 hour day-night cycle. The mechanisms generating circadian rhythms, their amplitude and circadian phase are dependent on a transcriptional network of immense complexity. Moreover, the contribution of post-transcriptional mechanisms in generating rhythms in RNA abundance is not known.   Results: Here, we analyzed the clock-controlled transcriptome of Neurospora crassa together with temporal profiles of elongating RNA polymerase II. Our data indicate that transcription contributes to the rhythmic expression of the vast majority of clock-controlled genes (ccgs) in Neurospora. The ccgs accumulate in two main clusters with peak transcription and expression levels either at dawn or dusk. Dawn-phased genes are predominantly involved in catabolic and dusk-phased genes in anabolic processes, indicating a clock-controlled temporal separation of the physiology of Neurospora. Genes whose expression is strongly dependent on the core circadian activator WCC fall mainly into the dawn-phased cluster while rhythmic genes regulated by the glucose-dependent repressor CSP1 fall predominantly into the dusk-phased cluster. Surprisingly, the number of rhythmic transcripts increases about twofold in the absence of CSP1, indicating that rhythmic expression of many genes is attenuated by the activity of CSP1.   Conclusions: The data indicate that the vast majority of transcript rhythms in Neurospora are generated by dawn and dusk specific transcription. Our observations suggest a substantial plasticity of the circadian transcriptome with respect to the number of rhythmic genes as well as amplitude and phase of the expression rhythms and emphasize a major role of the circadian clock in the temporal organization of metabolism and physiology.   
date: 2015
publisher: Springer
id_scheme: DOI
ppn_swb: 1653720182
own_urn: urn:nbn:de:bsz:16-heidok-194350
language: eng
bibsort: SANCARCIGDDAWNANDDUS2015
full_text_status: public
publication: BMC Biology
volume: 13
number: 17
place_of_pub: Berlin ; Heidelberg
pagerange: 1-16
issn: 1741-7007
citation:   Sancar, Cigdem ; Sancar, Gencer ; Ha, Nati ; Cesbron, François ; Brunner, Michael  (2015) Dawn- and dusk-phased circadian transcription rhythms coordinate anabolic and catabolic functions in Neurospora.  BMC Biology, 13 (17).  pp. 1-16.  ISSN 1741-7007     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19435/1/12915_2015_Article_126.pdf