eprintid: 19452
rev_number: 14
eprint_status: archive
userid: 1589
dir: disk0/00/01/94/52
datestamp: 2016-01-14 09:47:50
lastmod: 2024-03-09 08:58:05
status_changed: 2016-01-14 09:47:50
type: article
metadata_visibility: show
creators_name: Kansy, Benjamin A.
creators_name: Dißmann, Philip A.
creators_name: Hemeda, Hatim
creators_name: Bruderek, Kirsten
creators_name: Westerkamp, Anna M.
creators_name: Jagalski, Vivien
creators_name: Schuler, Patrick
creators_name: Kansy, Katinka
creators_name: Lang, Stephan
creators_name: Dumitru, Claudia A.
creators_name: Brandau, Sven
title: The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer
subjects: ddc-610
divisions: i-910800
abstract: Introduction: Mesenchymal stromal cells (MSC) are an integral cellular component of the tumor microenvironment. Nevertheless, very little is known about MSC originating from human malignant tissue and modulation of these cells by tumor-derived factors. The aim of this study was to isolate and characterize MSC from head and neck squamous cell carcinoma (HNSCC) and to investigate their interaction with tumor cells.   Methods: MSC were isolated from tumor tissues of HNSCC patients during routine oncological surgery. Immunophenotyping, immunofluorescence and in vitro differentiation were performed to determine whether the isolated cells met the consensus criteria for MSC. The cytokine profile of tumor-derived MSC was determined by enzyme-linked immunosorbent assay (ELISA). Activation of MSC by tumor-conditioned media was assessed by measuring cytokine release and expression of CD54. The impact of MSC on tumor growth in vivo was analyzed in a HNSCC xenograft model.   Results: Cells isolated from HNSCC tissue met the consensus criteria for MSC. Tumor-derived MSC constitutively produced high amounts of interleukin (IL)-6, IL-8 and stromal cell-derived factor (SDF)-1α. HNSCC-derived factors activated MSC and enhanced secretion of IL-8 and expression of CD54. Furthermore, MSC provided stromal support for human HNSCC cell lines in vivo and enhanced their growth in a murine xenograft model.   Conclusions: This is the first study to isolate and characterize MSC from malignant tissues of patients with HNSCC. We observed cross-talk of stromal cells and tumor cells resulting in enhanced growth of HNSCC in vivo.  
date: 2014
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1653736224
own_urn: urn:nbn:de:bsz:16-heidok-194522
language: eng
bibsort: KANSYBENJATHEBIDIREC2014
full_text_status: public
publication: Stem Cell Research & Therapy
volume: 5
number: 95
place_of_pub: London
pagerange: 1-10
issn: 1757-6512
citation:   Kansy, Benjamin A. ; Dißmann, Philip A. ; Hemeda, Hatim ; Bruderek, Kirsten ; Westerkamp, Anna M. ; Jagalski, Vivien ; Schuler, Patrick ; Kansy, Katinka ; Lang, Stephan ; Dumitru, Claudia A. ; Brandau, Sven  (2014) The bidirectional tumor - mesenchymal stromal cell interaction promotes the progression of head and neck cancer.  Stem Cell Research & Therapy, 5 (95).  pp. 1-10.  ISSN 1757-6512     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19452/1/13287_2013_Article_404.pdf