eprintid: 19557 rev_number: 12 eprint_status: archive userid: 1589 dir: disk0/00/01/95/57 datestamp: 2016-01-25 08:07:14 lastmod: 2024-05-08 23:19:25 status_changed: 2016-01-25 08:07:14 type: article metadata_visibility: show creators_name: Veres, Gábor creators_name: Radovits, Tamás creators_name: Seres, Leila creators_name: Horkay, Ferenc creators_name: Karck, Matthias creators_name: Szabó, Gábor title: Effects of inosine on reperfusion injury after cardiopulmonary bypass subjects: ddc-610 divisions: i-910200 abstract: Objective: Inosine, a break-down product of adenosine has been recently shown to exert inodilatory and anti-inflammatory properties. Furthermore inosine might be a key substrate of pharmacological post-conditioning. In the present pre-clinical study, we investigated the effects of inosine on cardiac function during reperfusion in an experimental model of cardioplegic arrest and extracorporal circulation. Methods: Twelve anesthetized dogs underwent hypothermic cardiopulmonary bypass. After 60 minutes of hypothermic cardiac arrest, reperfusion was started after application of either saline vehicle (control, n = 6), or inosine (100 mg/kg, n = 6). Left ventricular end-systolic pressure volume relationship (ESPVR) was measured by a combined pressure-volume-conductance catheter at baseline and after 60 minutes of reperfusion. Left anterior descendent coronary blood flow (CBF), endothelium-dependent vasodilatation to acetylcholine (ACh) and endothelium-independent vasodilatation to sodium nitroprusside (SNP) were also determined. Results: The administration of inosine led to a significantly better recovery (given as percent of baseline) of ESPVR 90 ± 9% vs. 46 ± 6%, p < 0.05. CBF and was also significantly higher in the inosine group (56 ± 8 vs. 23 ± 4, ml/min, p < 0.05). While the vasodilatatory response to SNP was similar in both groups, ACh resulted in a significantly higher increase in CBF (58 ± 6% vs. 25 ± 5%, p < 0.05) in the inosine group. Conclusions: Application of inosine improves myocardial and endothelial function after cardiopulmonary bypass with hypothermic cardiac arrest. date: 2010 publisher: BioMed Central id_scheme: DOI ppn_swb: 1653720557 own_urn: urn:nbn:de:bsz:16-heidok-195578 language: eng bibsort: VERESGABOREFFECTSOFI2010 full_text_status: public publication: Journal of Cardiothoracic Surgery volume: 5 number: 106 place_of_pub: London pagerange: 1-6 issn: 1749-8090 citation: Veres, Gábor ; Radovits, Tamás ; Seres, Leila ; Horkay, Ferenc ; Karck, Matthias ; Szabó, Gábor (2010) Effects of inosine on reperfusion injury after cardiopulmonary bypass. Journal of Cardiothoracic Surgery, 5 (106). pp. 1-6. ISSN 1749-8090 document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19557/1/13019_2010_Article_338.pdf