title: Gene expression of inflammasome components in peripheral blood mononuclear cells (PBMC) of vascular patients increases with age
creator: Wu, Xiaoyu
creator: Hakimi, Maani
creator: Wortmann, Markus
creator: Zhang, Jian
creator: Böckler, Dittmar
creator: Dihlmann, Susanne
subject: 610
subject: 610 Medical sciences Medicine
description: Background: Chronic low-grade inflammation is considered a driver of many age-related disorders, including vascular diseases (inflammaging). Inhibition of autophagic capacity with ageing was postulated to generate a pro-inflammatory condition via activation of inflammasomes, a group of Interleukin-1 activating intracellular multi-protein complexes. We thus investigated gene expression of inflammasome components in PBMC of 77 vascular patients (age 22–82) in association with age.   Findings: Linear regression of real-time qRT-PCR data revealed a significant positive association of gene expression of each of the inflammasome components with age (Pearson correlation coefficients: AIM2: r = 0.245; P = 0.032; NLRP3: r = 0.367; P = 0.001; ASC (PYCARD): r = 0.252; P = 0.027; CASP1: r = 0.296; P = 0.009; CASP5: r = 0.453; P = 0.00003; IL1B: r = 0.247; P = 0.030). No difference in gene expression of AIM2, NLRP3, ASC CASP1, and CASP5 was detected between PBMC of patients with advanced atherosclerosis and other vascular patients, whereas IL1B expression was increased in PBMC of the latter group (P = 0.0005).   Conclusion: The findings reinforce the systemic pro-inflammatory phenotype reported in elderly by demonstrating an increased phase-1 activation of inflammasomes in PBMC of vascular patients.  
publisher: BioMed Central
date: 2015
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/19583/1/12979_2015_Article_43.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-195832
identifier:   Wu, Xiaoyu ; Hakimi, Maani ; Wortmann, Markus ; Zhang, Jian ; Böckler, Dittmar ; Dihlmann, Susanne  (2015) Gene expression of inflammasome components in peripheral blood mononuclear cells (PBMC) of vascular patients increases with age.  Immunity & Ageing, 12 (15).  pp. 1-5.  ISSN 1742-4933     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/19583/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng