eprintid: 19583
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/01/95/83
datestamp: 2016-01-25 08:35:22
lastmod: 2022-07-09 21:47:39
status_changed: 2016-01-25 08:35:22
type: article
metadata_visibility: show
creators_name: Wu, Xiaoyu
creators_name: Hakimi, Maani
creators_name: Wortmann, Markus
creators_name: Zhang, Jian
creators_name: Böckler, Dittmar
creators_name: Dihlmann, Susanne
title: Gene expression of inflammasome components in peripheral blood mononuclear cells (PBMC) of vascular patients increases with age
subjects: 610
divisions: 910200
abstract: Background: Chronic low-grade inflammation is considered a driver of many age-related disorders, including vascular diseases (inflammaging). Inhibition of autophagic capacity with ageing was postulated to generate a pro-inflammatory condition via activation of inflammasomes, a group of Interleukin-1 activating intracellular multi-protein complexes. We thus investigated gene expression of inflammasome components in PBMC of 77 vascular patients (age 22–82) in association with age.   Findings: Linear regression of real-time qRT-PCR data revealed a significant positive association of gene expression of each of the inflammasome components with age (Pearson correlation coefficients: AIM2: r = 0.245; P = 0.032; NLRP3: r = 0.367; P = 0.001; ASC (PYCARD): r = 0.252; P = 0.027; CASP1: r = 0.296; P = 0.009; CASP5: r = 0.453; P = 0.00003; IL1B: r = 0.247; P = 0.030). No difference in gene expression of AIM2, NLRP3, ASC CASP1, and CASP5 was detected between PBMC of patients with advanced atherosclerosis and other vascular patients, whereas IL1B expression was increased in PBMC of the latter group (P = 0.0005).   Conclusion: The findings reinforce the systemic pro-inflammatory phenotype reported in elderly by demonstrating an increased phase-1 activation of inflammasomes in PBMC of vascular patients.  
date: 2015
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1653712732
own_urn: urn:nbn:de:bsz:16-heidok-195832
language: eng
bibsort: WUXIAOYUGENEEXPRES2015
full_text_status: public
publication: Immunity & Ageing
volume: 12
number: 15
place_of_pub: London
pagerange: 1-5
issn: 1742-4933
citation:   Wu, Xiaoyu ; Hakimi, Maani ; Wortmann, Markus ; Zhang, Jian ; Böckler, Dittmar ; Dihlmann, Susanne  (2015) Gene expression of inflammasome components in peripheral blood mononuclear cells (PBMC) of vascular patients increases with age.  Immunity & Ageing, 12 (15).  pp. 1-5.  ISSN 1742-4933     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19583/1/12979_2015_Article_43.pdf