title: A robust potency assay highlights significant donor variation of human mesenchymal stem/progenitor cell immune modulatory capacity and extended radio-resistance
creator: Ketterl, Nina
creator: Brachtl, Gabriele
creator: Schuh, Cornelia
creator: Bieback, Karen
creator: Schallmoser, Katharina
creator: Reinisch, Andreas
creator: Strunk, Dirk
subject: ddc-570
subject: 570 Life sciences
description: The inherent immunomodulatory capacity of mesenchymal stem/progenitor cells (MSPCs) encouraged initiation of multiple clinical trials. Release criteria for therapeutic MSPCs cover identity, purity and safety but appropriate potency assessment is often missing. Reports on functional heterogeneity of MSPCs created additional uncertainty regarding donor and organ/source selection. We established a robust immunomodulation potency assay based on pooling responder leukocytes to minimize individual immune response variability. Comparing various MSPCs revealed significant potency inconsistency and generally diminished allo-immunosuppression compared to dose-dependent inhibition of mitogenesis. Gamma-irradiation to block unintended MSPC proliferation did not prohibit chondrogenesis and osteogenesis in vivo, indicating the need for alternative safety strategies.
publisher: BioMed Central
date: 2015
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/19878/1/13287_2015_Article_233.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-198781
identifier:   Ketterl, Nina ; Brachtl, Gabriele ; Schuh, Cornelia ; Bieback, Karen ; Schallmoser, Katharina ; Reinisch, Andreas ; Strunk, Dirk  (2015) A robust potency assay highlights significant donor variation of human mesenchymal stem/progenitor cell immune modulatory capacity and extended radio-resistance.  Stem Cell Research & Therapy, 6 (236).  pp. 1-11.  ISSN 1757-6512     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/19878/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng