eprintid: 19878
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/01/98/78
datestamp: 2016-02-08 13:59:35
lastmod: 2024-05-25 16:18:41
status_changed: 2016-02-08 13:59:35
type: article
metadata_visibility: show
creators_name: Ketterl, Nina
creators_name: Brachtl, Gabriele
creators_name: Schuh, Cornelia
creators_name: Bieback, Karen
creators_name: Schallmoser, Katharina
creators_name: Reinisch, Andreas
creators_name: Strunk, Dirk
title: A robust potency assay highlights significant donor variation of human mesenchymal stem/progenitor cell immune modulatory capacity and extended radio-resistance
subjects: ddc-570
divisions: i-63400
abstract: The inherent immunomodulatory capacity of mesenchymal stem/progenitor cells (MSPCs) encouraged initiation of multiple clinical trials. Release criteria for therapeutic MSPCs cover identity, purity and safety but appropriate potency assessment is often missing. Reports on functional heterogeneity of MSPCs created additional uncertainty regarding donor and organ/source selection. We established a robust immunomodulation potency assay based on pooling responder leukocytes to minimize individual immune response variability. Comparing various MSPCs revealed significant potency inconsistency and generally diminished allo-immunosuppression compared to dose-dependent inhibition of mitogenesis. Gamma-irradiation to block unintended MSPC proliferation did not prohibit chondrogenesis and osteogenesis in vivo, indicating the need for alternative safety strategies.
date: 2015
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1655245406
own_urn: urn:nbn:de:bsz:16-heidok-198781
language: eng
bibsort: KETTERLNINAROBUSTPOT2015
full_text_status: public
publication: Stem Cell Research & Therapy
volume: 6
number: 236
place_of_pub: London
pagerange: 1-11
issn: 1757-6512
citation:   Ketterl, Nina ; Brachtl, Gabriele ; Schuh, Cornelia ; Bieback, Karen ; Schallmoser, Katharina ; Reinisch, Andreas ; Strunk, Dirk  (2015) A robust potency assay highlights significant donor variation of human mesenchymal stem/progenitor cell immune modulatory capacity and extended radio-resistance.  Stem Cell Research & Therapy, 6 (236).  pp. 1-11.  ISSN 1757-6512     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19878/1/13287_2015_Article_233.pdf