title: Geometric constrains for detecting short actin filaments by cryogenic electron tomography
creator: Kudryashev, Mikhail
creator: Lepper, Simone
creator: Baumeister, Wolfgang
creator: Cyrklaff, Marek
creator: Frischknecht, Friedrich
subject: ddc-570
subject: 570 Life sciences
description: Polymerization of actin into filaments can push membranes forming extensions like filopodia or lamellipodia, which are important during processes such as cell motility and phagocytosis. Similarly, small organelles or pathogens can be moved by actin polymerization. Such actin filaments can be arranged in different patterns and are usually hundreds of nanometers in length as revealed by various electron microscopy approaches. Much shorter actin filaments are involved in the motility of apicomplexan parasites. However, these short filaments have to date not been visualized in intact cells. Here, we investigated Plasmodium sporozoites, the motile forms of the malaria parasite that are transmitted by the mosquito, using cryogenic electron tomography. We detected filopodia-like extensions of the plasma membrane and observed filamentous structures in the supra-alveolar space underneath the plasma membrane. However, these filaments could not be unambiguously assigned as actin filaments. In silico simulations of EM data collection and tomographic reconstruction identify the limits in revealing the filaments due to their length, concentration and orientation. PACS Codes: 87.64.Ee
publisher: Springer; BioMed Central
date: 2010
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/19916/1/13074_2009_Article_24.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-199160
identifier:   Kudryashev, Mikhail ; Lepper, Simone ; Baumeister, Wolfgang ; Cyrklaff, Marek ; Frischknecht, Friedrich  (2010) Geometric constrains for detecting short actin filaments by cryogenic electron tomography.  PMC Biophysics, 3 (6).  pp. 1-14.  ISSN 1757-5036     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/19916/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng