eprintid: 19916
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/01/99/16
datestamp: 2016-02-09 08:26:57
lastmod: 2024-05-09 15:30:02
status_changed: 2016-02-09 08:26:57
type: article
metadata_visibility: show
creators_name: Kudryashev, Mikhail
creators_name: Lepper, Simone
creators_name: Baumeister, Wolfgang
creators_name: Cyrklaff, Marek
creators_name: Frischknecht, Friedrich
title: Geometric constrains for detecting short actin filaments by cryogenic electron tomography
subjects: ddc-570
divisions: i-718000
divisions: i-911700
abstract: Polymerization of actin into filaments can push membranes forming extensions like filopodia or lamellipodia, which are important during processes such as cell motility and phagocytosis. Similarly, small organelles or pathogens can be moved by actin polymerization. Such actin filaments can be arranged in different patterns and are usually hundreds of nanometers in length as revealed by various electron microscopy approaches. Much shorter actin filaments are involved in the motility of apicomplexan parasites. However, these short filaments have to date not been visualized in intact cells. Here, we investigated Plasmodium sporozoites, the motile forms of the malaria parasite that are transmitted by the mosquito, using cryogenic electron tomography. We detected filopodia-like extensions of the plasma membrane and observed filamentous structures in the supra-alveolar space underneath the plasma membrane. However, these filaments could not be unambiguously assigned as actin filaments. In silico simulations of EM data collection and tomographic reconstruction identify the limits in revealing the filaments due to their length, concentration and orientation. PACS Codes: 87.64.Ee
date: 2010
publisher: Springer; BioMed Central
id_scheme: DOI
ppn_swb: 1655516213
own_urn: urn:nbn:de:bsz:16-heidok-199160
language: eng
bibsort: KUDRYASHEVGEOMETRICC2010
full_text_status: public
publication: PMC Biophysics
volume: 3
number: 6
place_of_pub: Berlin; Heidelberg; London
pagerange: 1-14
issn: 1757-5036
citation:   Kudryashev, Mikhail ; Lepper, Simone ; Baumeister, Wolfgang ; Cyrklaff, Marek ; Frischknecht, Friedrich  (2010) Geometric constrains for detecting short actin filaments by cryogenic electron tomography.  PMC Biophysics, 3 (6).  pp. 1-14.  ISSN 1757-5036     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/19916/1/13074_2009_Article_24.pdf