title: RNA degradation compromises the reliability of microRNA expression profiling
creator: Ibberson, David
creator: Benes, Vladimir
creator: Muckenthaler, Martina U.
creator: Castoldi, Mirco
subject: ddc-570
subject: 570 Life sciences
description: Background: MicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression and their expression is frequently altered in human diseases, including cancer. To correlate clinically relevant parameters with microRNA expression, total RNA is frequently prepared from samples that were archived for various time periods in frozen tissue banks but, unfortunately, RNA integrity is not always preserved in these frozen tissues. Here, we investigate whether experimentally induced RNA degradation affects microRNA expression profiles.   Results: Tissue samples were maintained on ice for defined time periods prior to total RNA extraction, which resulted in different degrees of RNA degradation. MicroRNA expression was then analyzed by microarray analysis (miCHIP) or microRNA-specific real-time quantitative PCR (miQPCR). Our results demonstrate that the loss of RNA integrity leads to in unpredictability of microRNA expression profiles for both, array-based and miQPCR assays.   Conclusion: MicroRNA expression cannot be reliably profiled in degraded total RNA. For the profiling of microRNAs we recommend use of RNA samples with a RNA integrity number equal to or above seven.
publisher: BioMed Central
date: 2009
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/19930/1/12896_2009_Article_468.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-199308
identifier:   Ibberson, David ; Benes, Vladimir ; Muckenthaler, Martina U. ; Castoldi, Mirco  (2009) RNA degradation compromises the reliability of microRNA expression profiling.  BMC Biotechnology, 9 (102).  pp. 1-9.  ISSN 1472-6750     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/19930/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng