<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Development of Droplet-Based Microfluidics for Synthetic Biology\r\nApplications"^^ . "Microfluidics combines principles of science and technology, and enables the user to handle,\r\nprocess and manipulate fluids of very small volumes. This technology permits the\r\nintegration of multiple laboratory applications into one single microfabricated chip, requires\r\nminimal manual user intervention and sample consumption, and allows enhanced\r\ndata analysis speed and precision. Due to these numerous advantages, the potential for\r\nthis technology to be applied in fundamental biophysical and biomedical research is vast.\r\nThe major aim of this thesis was to explore the capacities of microfluidics, particularly\r\ndroplet-based microfluidic technology in the following topics: 1) Mimicry of the immune\r\nsystem cellular environment, with the ultimate goal of programing T cells for adoptive\r\nT cell therapy; 2) Bottom-up assembly of minimal synthetic cells. Towards this end, a\r\nnovel approach to form gold-nanostructured and specifically biofunctionalized water-in-oil\r\ndroplets was developed. This thesis highlights the advanced properties of nanostructured\r\ndroplets to serve as 3D antigen presenting cell (APC) surrogates for T-cell stimulation.\r\nThe combination of flexible biofunctionalization and pliable physical droplet properties\r\nwork in tandem, providing a flexible and modular system that closely models in situ\r\nAPC-T cell interactions. The research within this thesis focused also on the dissection of\r\ncomplex cellular sensory machinery implementing an automated droplet-based microfluidic\r\napproach. Towards this goal, nanostructured droplets as cell-sized compartments and\r\ndroplet-based pico-injection technology were used to achieve the bottom-up assembly of\r\nthe minimal number of proteins required for a “simple synthetic cell.” While the applied\r\nmethodology has a potential for assembly of a wide range of subcellular functional units,\r\nthe focus in this thesis was on the reconstitution of the actomyosin cortex. Successful\r\noptimization of the biochemical and biophysical conditions within the droplets allowed to\r\nachieve precise control over the actin polymerization and actomyosin network organization\r\nby their linkage to the droplets periphery. These experimental steps were also necessary\r\nto generate signaling events including myosin-driven droplet migration and self-propulsion\r\nwith reduced molecular complexity compared to living cells."^^ . "2015" . . . . . . . "Jan-Willi"^^ . "Janiesch"^^ . "Jan-Willi Janiesch"^^ . . . . . . "Development of Droplet-Based Microfluidics for Synthetic Biology\r\nApplications (PDF)"^^ . . . "Janieschthesis.pdf"^^ . . . "Development of Droplet-Based Microfluidics for Synthetic Biology\r\nApplications (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Development of Droplet-Based Microfluidics for Synthetic Biology\r\nApplications (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Development of Droplet-Based Microfluidics for Synthetic Biology\r\nApplications (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Development of Droplet-Based Microfluidics for Synthetic Biology\r\nApplications (Other)"^^ . . . . . . "small.jpg"^^ . . . "Development of Droplet-Based Microfluidics for Synthetic Biology\r\nApplications (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #19990 \n\nDevelopment of Droplet-Based Microfluidics for Synthetic Biology \nApplications\n\n" . "text/html" . . . "540 Chemie"@de . "540 Chemistry and allied sciences"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . . . "600 Technik, Medizin, angewandte Wissenschaften"@de . "600 Technology (Applied sciences)"@en . .