eprintid: 20059
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/00/59
datestamp: 2016-02-15 14:11:27
lastmod: 2024-04-27 13:22:31
status_changed: 2016-02-15 14:11:27
type: article
metadata_visibility: show
creators_name: Mulindwa, Julius
creators_name: Mercé, Clémentine
creators_name: Matovu, Enock
creators_name: Enyaru, John
creators_name: Clayton, Christine
title: Transcriptomes of newly-isolated Trypanosoma brucei rhodesiense reveal hundreds of mRNAs that are co-regulated with stumpy-form markers
subjects: 570
divisions: 706000
divisions: 718000
abstract: Background: During natural Trypanosoma brucei infections, the parasites differentiate spontaneously into a non-dividing “stumpy” form when a certain level of parasitaemia is attained. This form is metabolically adapted for rapid further differentiation into procyclic forms upon uptake by Tsetse flies.   Results: We describe here four central Ugandan isolates of Trypanosoma brucei rhodesiense that have undergone only three rodent passages since isolation from human patients. As expected, SNP analysis shows that these isolates are more closely related to each other than to the commonly used strains Lister 427, Antat1.1, and TREU927. TREU927 generally has smaller copy numbers of repeated genes than the other strains, while Lister 427 trypanosomes with a 30-year history of in vitro culture and cloning have more histone genes than the other isolates. The recently isolated trypanosomes were grown in rats, and their transcriptomes characterised. In comparison with cultured procyclic and bloodstream forms, there were increases in mRNAs encoding the stumpy-form markers ESAG9 and PIP39, with coordinated alterations in the levels of over 600 additional mRNAs. Numerous mRNAs encoding proteins of no known function were either increased or decreased. The products of the mRNAs that were increased in parallel with PIP39 included not only enzymes of procyclic-form metabolism, but also components of the translational and RNA control machineries. Many of the mRNAs that were decreased in cells with elevated PIP39 reflected reduced cell division.   Conclusions: These transcriptomes suggest new avenues for research into the regulation of trypanosome differentiation.
date: 2015
publisher: BioMed Central; Springer
id_scheme: DOI
ppn_swb: 1657159140
own_urn: urn:nbn:de:bsz:16-heidok-200593
language: eng
bibsort: MULINDWAJUTRANSCRIPT2015
full_text_status: public
publication: BMC Genomics
volume: 16
number: 1118
place_of_pub: London; Berlin; Heidelberg
pagerange: 1-10
issn: 1471-2164
citation:   Mulindwa, Julius ; Mercé, Clémentine ; Matovu, Enock ; Enyaru, John ; Clayton, Christine  (2015) Transcriptomes of newly-isolated Trypanosoma brucei rhodesiense reveal hundreds of mRNAs that are co-regulated with stumpy-form markers.  BMC Genomics, 16 (1118).  pp. 1-10.  ISSN 1471-2164     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/20059/1/12864_2015_Article_2338.pdf