title: Deleted in Malignant Brain Tumors 1 (DMBT1) is present in hyaline membranes and modulates surface tension of surfactant
creator: Müller, Hanna
creator: End, Caroline
creator: Renner, Marcus
creator: Helmke, Burkhard M
creator: Gassler, Nikolaus
creator: Weiss, Christel
creator: Hartl, Dominik
creator: Griese, Matthias
creator: Hafner, Mathias
creator: Poustka, Annemarie
creator: Mollenhauer, Jan
creator: Pöschl, Johannes
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Background: Deleted in Malignant Brain Tumors 1 (DMBT1) is a secreted scavenger receptor cysteine-rich protein that binds various bacteria and is thought to participate in innate pulmonary host defense. We hypothesized that pulmonary DMBT1 could contribute to respiratory distress syndrome in neonates by modulating surfactant function.   Methods: DMBT1 expression was studied by immunohistochemistry and mRNA in situ hybridization in post-mortem lungs of preterm and full-term neonates with pulmonary hyaline membranes. The effect of human recombinant DMBT1 on the function of bovine and porcine surfactant was measured by a capillary surfactometer. DMBT1-levels in tracheal aspirates of ventilated preterm and term infants were determined by ELISA.   Results: Pulmonary DMBT1 was localized in hyaline membranes during respiratory distress syndrome. In vitro addition of human recombinant DMBT1 to the surfactants increased surface tension in a dose-dependent manner. The DMBT1-mediated effect was reverted by the addition of calcium depending on the surfactant preparation.   Conclusion: Our data showed pulmonary DMBT1 expression in hyaline membranes during respiratory distress syndrome and demonstrated that DMBT1 increases lung surface tension in vitro. This raises the possibility that DMBT1 could antagonize surfactant supplementation in respiratory distress syndrome and could represent a candidate target molecule for therapeutic intervention in neonatal lung disease.
publisher: BioMed Central
date: 2007
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/20064/1/12931_2007_Article_607.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-200644
identifier:   Müller, Hanna ; End, Caroline ; Renner, Marcus ; Helmke, Burkhard M ; Gassler, Nikolaus ; Weiss, Christel ; Hartl, Dominik ; Griese, Matthias ; Hafner, Mathias ; Poustka, Annemarie ; Mollenhauer, Jan ; Pöschl, Johannes  (2007) Deleted in Malignant Brain Tumors 1 (DMBT1) is present in hyaline membranes and modulates surface tension of surfactant.  Respiratory Research, 8 (69).  pp. 1-10.  ISSN 1465-993X     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/20064/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng