eprintid: 20064
rev_number: 14
eprint_status: archive
userid: 1589
dir: disk0/00/02/00/64
datestamp: 2016-02-15 14:32:25
lastmod: 2024-05-03 19:57:05
status_changed: 2016-02-15 14:32:25
type: article
metadata_visibility: show
creators_name: Müller, Hanna
creators_name: End, Caroline
creators_name: Renner, Marcus
creators_name: Helmke, Burkhard M
creators_name: Gassler, Nikolaus
creators_name: Weiss, Christel
creators_name: Hartl, Dominik
creators_name: Griese, Matthias
creators_name: Hafner, Mathias
creators_name: Poustka, Annemarie
creators_name: Mollenhauer, Jan
creators_name: Pöschl, Johannes
title: Deleted in Malignant Brain Tumors 1 (DMBT1) is present in hyaline membranes and modulates surface tension of surfactant
subjects: 610
divisions: 63000
divisions: 850300
divisions: 910500
divisions: 912000
abstract: Background: Deleted in Malignant Brain Tumors 1 (DMBT1) is a secreted scavenger receptor cysteine-rich protein that binds various bacteria and is thought to participate in innate pulmonary host defense. We hypothesized that pulmonary DMBT1 could contribute to respiratory distress syndrome in neonates by modulating surfactant function.   Methods: DMBT1 expression was studied by immunohistochemistry and mRNA in situ hybridization in post-mortem lungs of preterm and full-term neonates with pulmonary hyaline membranes. The effect of human recombinant DMBT1 on the function of bovine and porcine surfactant was measured by a capillary surfactometer. DMBT1-levels in tracheal aspirates of ventilated preterm and term infants were determined by ELISA.   Results: Pulmonary DMBT1 was localized in hyaline membranes during respiratory distress syndrome. In vitro addition of human recombinant DMBT1 to the surfactants increased surface tension in a dose-dependent manner. The DMBT1-mediated effect was reverted by the addition of calcium depending on the surfactant preparation.   Conclusion: Our data showed pulmonary DMBT1 expression in hyaline membranes during respiratory distress syndrome and demonstrated that DMBT1 increases lung surface tension in vitro. This raises the possibility that DMBT1 could antagonize surfactant supplementation in respiratory distress syndrome and could represent a candidate target molecule for therapeutic intervention in neonatal lung disease.
date: 2007
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1656587009
own_urn: urn:nbn:de:bsz:16-heidok-200644
language: eng
bibsort: MULLERHANNDELETEDINM2007
full_text_status: public
publication: Respiratory Research
volume: 8
number: 69
place_of_pub: London
pagerange: 1-10
issn: 1465-993X
citation:   Müller, Hanna ; End, Caroline ; Renner, Marcus ; Helmke, Burkhard M ; Gassler, Nikolaus ; Weiss, Christel ; Hartl, Dominik ; Griese, Matthias ; Hafner, Mathias ; Poustka, Annemarie ; Mollenhauer, Jan ; Pöschl, Johannes  (2007) Deleted in Malignant Brain Tumors 1 (DMBT1) is present in hyaline membranes and modulates surface tension of surfactant.  Respiratory Research, 8 (69).  pp. 1-10.  ISSN 1465-993X     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/20064/1/12931_2007_Article_607.pdf