eprintid: 20127
rev_number: 13
eprint_status: archive
userid: 1589
dir: disk0/00/02/01/27
datestamp: 2016-02-22 12:16:17
lastmod: 2024-03-24 00:37:42
status_changed: 2016-02-22 12:16:17
type: article
metadata_visibility: show
creators_name: Pandey, Priyanka
creators_name: Brors, Benedikt
creators_name: Srivastava, Prashant K.
creators_name: Bott, Andrea
creators_name: Böhn, Susanne NE
creators_name: Gröne, Herrmann-Josef
creators_name: Gretz, Norbert
title: Microarray-based approach identifies microRNAs and their target functional patterns in polycystic kidney disease
subjects: ddc-610
divisions: i-999811
divisions: i-850300
abstract: Background: MicroRNAs (miRNAs) play key roles in mammalian gene expression and several cellular processes, including differentiation, development, apoptosis and cancer pathomechanisms. Recently the biological importance of primary cilia has been recognized in a number of human genetic diseases. Numerous disorders are related to cilia dysfunction, including polycystic kidney disease (PKD). Although involvement of certain genes and transcriptional networks in PKD development has been shown, not much is known how they are regulated molecularly.   Results: Given the emerging role of miRNAs in gene expression, we explored the possibilities of miRNA-based regulations in PKD. Here, we analyzed the simultaneous expression changes of miRNAs and mRNAs by microarrays. 935 genes, classified into 24 functional categories, were differentially regulated between PKD and control animals. In parallel, 30 miRNAs were differentially regulated in PKD rats: our results suggest that several miRNAs might be involved in regulating genetic switches in PKD. Furthermore, we describe some newly detected miRNAs, miR-31 and miR-217, in the kidney which have not been reported previously. We determine functionally related gene sets, or pathways to reveal the functional correlation between differentially expressed mRNAs and miRNAs.   Conclusion: We find that the functional patterns of predicted miRNA targets and differentially expressed mRNAs are similar. Our results suggest an important role of miRNAs in specific pathways underlying PKD.
date: 2008
publisher: BioMed Central; Springer
id_scheme: DOI
ppn_swb: 132616564
own_urn: urn:nbn:de:bsz:16-heidok-201272
language: eng
bibsort: PANDEYPRIYMICROARRAY2008
full_text_status: public
publication: BMC Genomics
volume: 9
number: 624
place_of_pub: London; Berlin; Heidelberg
pagerange: 1-17
issn: 1471-2164
citation:   Pandey, Priyanka ; Brors, Benedikt ; Srivastava, Prashant K. ; Bott, Andrea ; Böhn, Susanne NE ; Gröne, Herrmann-Josef ; Gretz, Norbert  (2008) Microarray-based approach identifies microRNAs and their target functional patterns in polycystic kidney disease.  BMC Genomics, 9 (624).  pp. 1-17.  ISSN 1471-2164     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/20127/1/12864_2008_Article_1817.pdf