<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Multicolour STED nanoscopy with hyperspectral detection"^^ . "Within the scope of this thesis, a new multicolour STED microscope with a hyperspectral detection was designed and built. The challenges regarding the limitation of usable fluorophores as well as the stability of the optical set-up were overcome. Different existing spectral unmixing methods were compared to a self-implemented algorithm, both in simulations and using experimental data. A detailed study of the best separation conditions for several dye combinations was carried out for each of the methods. During the experiments, a spectral blue shift of several dyes was observed and its mechanisms were investigated. The optical resolution of the set-up was dye dependent and better than 80nm for all dyes. Moreover, an excellent separation for three fluorescent bead species and good a separation for a four-colour fixed-cell sample was achieved. This was done for the first time by purely distinguishing the different emission spectra using only one wavelength for excitation and a single STED beam for depletion."^^ . "2016" . . . . . . . "Franziska Ruth"^^ . "Winter"^^ . "Franziska Ruth Winter"^^ . . . . . . "Multicolour STED nanoscopy with hyperspectral detection (PDF)"^^ . . . "Dissertation_FranziskaWinter_Nov2015_v2.pdf"^^ . . . "Multicolour STED nanoscopy with hyperspectral detection (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Multicolour STED nanoscopy with hyperspectral detection (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Multicolour STED nanoscopy with hyperspectral detection (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Multicolour STED nanoscopy with hyperspectral detection (Other)"^^ . . . . . . "small.jpg"^^ . . . "Multicolour STED nanoscopy with hyperspectral detection (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #20199 \n\nMulticolour STED nanoscopy with hyperspectral detection\n\n" . "text/html" . . . "530 Physik"@de . "530 Physics"@en . .